ELISA/assay

Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial, CYP27B1 ELISA Kit

MBS937445

48-Strip-Wells

510 USD

ELISA Kit

Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial, CYP27B1 ELISA Kit

cytochrome P450, family 27, subfamily B, polypeptide 1

Human 25-hydroxyvitamin D-1 alpha hydroxylase; mitochondrial (CYP27B1) ELISA kit; CP2B; CYP1; CYP1alpha; CYP27B; P450c1; PDDR; VDD1; VDDR; VDDRI; VDR; 1alpha (OH) ase; 25 hydroxyvitamin D3-1-alpha hydroxylase; 25-Hydroxyvitamin D3 1alpha-hydroxylase; 25-OHD-1 alpha-hydroxylase; 25-hydr; cytochrome P450; family 27; subfamily B; polypeptide 1

25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial; 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial; 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial; 1alpha(OH)ase; VD3 1A hydroxylase; cytochrome p450 27B1; cytochrome P450C1 alpha; cytochrome P450VD1-alpha; calcidiol 1-monooxygenase; 25-OHD-1 alpha-hydroxylase; 25 hydroxyvitamin D3-1-alpha hydroxylase; cytochrome P450 subfamily XXVIIB polypeptide 1; cytochrome P450, family 27, subfamily B, polypeptide 1; 25-OHD-1 alpha-hydroxylase; 25-hydroxyvitamin D(3) 1-alpha-hydroxylase; VD3 1A hydroxylase; Calcidiol 1-monooxygenase; Cytochrome P450 subfamily XXVIIB polypeptide 1; Cytochrome P450C1 alpha; Cytochrome P450VD1-alpha; Cytochrome p450 27B1

CYP27B1

CYP27B1; CYP27B1; VDR; CP2B; CYP1; PDDR; VDD1; VDDR; VDDRI; CYP27B; P450c1; CYP1alpha; CYP1ALPHA; CYP27B; VD3 1A hydroxylase

CP27B_HUMAN

Human

508

This assay has high sensitivity and excellent specificity for detection of human CYP27B1. No significant cross-reactivity or interference between human CYP27B1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: 7.81 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYP27B1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYP27B1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP27B1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP27B1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4503213

NP_000776.1

NM_000785.3

O15528

56,504 Da

1,25-dihydroxyvitamin D3 Biosynthesis Pathway (545291); Biological Oxidations Pathway (105698); Cholecalciferol Biosynthesis Pathway (413391); Cholecalciferol Biosynthesis Pathway (468294); Cytochrome P450 - Arranged By Substrate Type Pathway (105700); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Steroid Hormones And Vitamins A And D Pathway (106150); Nuclear Receptors In Lipid Metabolism And Toxicity Pathway (198887); Phase 1 - Functionalization Of Compounds Pathway (105699)

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I. [provided by RefSeq, Jul 2008]

CYP27B1: Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation. Defects in CYP27B1 are the cause of rickets vitamin D- dependent type 1A (VDDR1A); also known as pseudovitamin D deficiency rickets (PDDR). A disorder caused by a selective deficiency of the active form of vitamin D (1,25- dihydroxyvitamin D3) and resulting in defective bone mineralization and clinical features of rickets. Belongs to the cytochrome P450 family. Protein type: Oxidoreductase; Mitochondrial; EC 1.14.13.13; Cell cycle regulation; Lipid Metabolism - steroid biosynthesis. Chromosomal Location of Human Ortholog: 12q14.1. Cellular Component: mitochondrial outer membrane; mitochondrion; cytoplasm. Molecular Function: iron ion binding; heme binding; calcidiol 1-monooxygenase activity. Biological Process: steroid metabolic process; vitamin metabolic process; response to lipopolysaccharide; decidualization; bone mineralization; vitamin D metabolic process; calcium ion homeostasis; negative regulation of cell proliferation; response to vitamin D; response to estrogen stimulus; xenobiotic metabolic process; calcium ion transport; negative regulation of cell growth; regulation of bone mineralization; positive regulation of keratinocyte differentiation; vitamin D catabolic process. Disease: Vitamin D Hydroxylation-deficient Rickets, Type 1a

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

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4800