catalog number :
MBS937414
products type :
ELISA Kit
products full name :
Horse Annexin A1, ANXA1 ELISA Kit
products short name :
annexin A1
products name syn :
Horse Annexin A1 (ANXA1) ELISA kit; ANX1; LPC1; OTTHUMP00000021475; annexin I; annexin I (lipocortin I) ; lipocortin I; annexin A1
other names :
annexin A1; Annexin A1; annexin A1; annexin I; annexin-1; calpactin-2; calpactin II; lipocortin I; lipocortin-1; ANXA1-like protein; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Lipocortin I
products gene name :
ANXA1
other gene names :
ANXA1; ANXA1; ANX1
uniprot entry name :
ANXA1_HORSE
specificity :
This assay has high sensitivity and excellent specificity for detection of horse ANXA1. No significant cross-reactivity or interference between horse ANXA1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 12.5 ng/ml -800 ng/ml. Sensitivity: The minimum detectable dose of horse ANXA1 is typically less than 3.12 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANXA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANXA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANXA1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANXA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001075336.1
ncbi gb acc num :
NM_001081867.2
ncbi mol weight :
38,737 Da
uniprot summary :
Function: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity
size4 :
10x96-Strip-Wells