product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat Secreted frizzled-related protein 1, SFRP1 ELISA Kit
catalog :
MBS937393
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS937393
products type :
ELISA Kit
products full name :
Rat Secreted frizzled-related protein 1, SFRP1 ELISA Kit
products short name :
secreted frizzled-related protein 1
products name syn :
Rat Secreted frizzled-related protein 1 (SFRP1) ELISA kit; FRP; FRP-1; FRP1; FrzA; SARP2; secreted apoptosis-related protein 2; secreted frizzled-related protein 1
other names :
Secreted frizzled-related protein 1; Secreted frizzled-related protein 1; secreted frizzled-related protein 1; secreted frizzled-related protein 1
products gene name :
SFRP1
other gene names :
Sfrp1; Sfrp1; sFRP-1; sFRP-1
uniprot entry name :
SFRP1_RAT
reactivity :
Rat
sequence length :
158
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat SFRP1. No significant cross-reactivity or interference between Rat SFRP1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.23 ng/ml-15 ng/ml. Sensitivity: 0.05 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Developmental Protein. Protein Biological Process 3: Differentiation
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SFRP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFRP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFRP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFRP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
61216364
ncbi acc num :
Q9R168.1
uniprot acc num :
Q9R168
ncbi mol weight :
18,072 Da
ncbi pathways :
Wnt Signaling Pathway NetPath (198488); Wnt Signaling Pathway (83453); Wnt Signaling Pathway (471)
ncbi summary :
a secreted protein involved in binding Wnt and antagonizing Wnt signaling [RGD, Feb 2006]
uniprot summary :
Function: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase. In kidney development, inhibits tubule formation and bud growth in metanephroi. Inhibits WNT1/WNT4-mediated TCF-dependent transcription.
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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