Human choline acetyltransterase, ChAT ELISA Kit | MyBioSource MBS937229 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human choline acetyltransterase, ChAT ELISA Kit

catalog :

MBS937229

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS937229

products type :

ELISA Kit

products full name :

Human choline acetyltransterase, ChAT ELISA Kit

products short name :

choline acetyltransferase

products name syn :

Human choline acetyltransterase (ChAT) ELISA Kit; CMS1A; CMS1A2; acetyl CoA:choline O-acetyltransferase; choline acetyltransferase

other names :

choline O-acetyltransferase isoform 1; Choline O-acetyltransferase; choline O-acetyltransferase; choline acetylase; acetyl CoA:choline O-acetyltransferase; choline O-acetyltransferase

products gene name :

CHAT

other gene names :

CHAT; CHAT; CMS1A; CMS1A2; CHOACTASE; CHOACTase; ChAT; Choline acetylase

uniprot entry name :

CLAT_HUMAN

reactivity :

Human

sequence length :

630

specificity :

This assay has high sensitivity and excellent specificity for detection of human ChAT. No significant cross-reactivity or interference between human ChAT and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of human ChAT is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ChAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ChAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ChAT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ChAT bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

218931221

ncbi acc num :

NP_001136401.1

ncbi gb acc num :

NM_001142929.1

uniprot acc num :

P28329

ncbi mol weight :

82,536 Da

ncbi pathways :

Acetylcholine Neurotransmitter Release Cycle Pathway (106525); Acetylcholine Synthesis Pathway (198820); Biogenic Amine Synthesis Pathway (198793); Cholinergic Synapse Pathway (217716); Glycerophospholipid Metabolism Pathway (82989); Glycerophospholipid Metabolism Pathway (364); Neuronal System Pathway (106513); Neurotransmitter Release Cycle Pathway (106518); Transmission Across Chemical Synapses Pathway (106516)

ncbi summary :

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]

uniprot summary :

CHAT: an enzyme that catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Four alternative splice variants have been described. Protein type: EC 2.3.1.6; Lipid Metabolism - glycerophospholipid; Acetyltransferase. Chromosomal Location of Human Ortholog: 10q11.2. Cellular Component: mitochondrion; cell soma; axon; cytoplasm; nucleus; cytosol. Molecular Function: choline O-acetyltransferase activity. Biological Process: synaptic transmission; rhythmic excitation; rhythmic behavior; muscle development; neurotransmitter secretion; phospholipid metabolic process; glycerophospholipid biosynthetic process; dendrite development; phosphatidylcholine biosynthetic process; neuromuscular synaptic transmission; establishment of synaptic specificity at neuromuscular junction; adult walking behavior; neurotransmitter biosynthetic process. Disease: Myasthenic Syndrome, Congenital, Associated With Episodic Apnea

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage