Human Extracellular superoxide dismutase [Cu-Zn], SOD3 ELISA Kit | MyBioSource MBS936860 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Extracellular superoxide dismutase [Cu-Zn], SOD3 ELISA Kit

catalog :

MBS936860

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS936860

products type :

ELISA Kit

products full name :

Human Extracellular superoxide dismutase [Cu-Zn], SOD3 ELISA Kit

products short name :

superoxide dismutase 3, extracellular

products name syn :

Human Extracellular superoxide dismutase [Cu-Zn] (SOD3) ELISA kit; EC-SOD; MGC20077; ; superoxide dismutase 3; extracellular

other names :

extracellular superoxide dismutase; Extracellular superoxide dismutase [Cu-Zn]; extracellular superoxide dismutase [Cu-Zn]; extracellular superoxide dismutase [Cu-Zn]; superoxide dismutase 3, extracellular

products gene name :

SOD3

other gene names :

SOD3; SOD3; EC-SOD; EC-SOD

uniprot entry name :

SODE_HUMAN

reactivity :

Human

sequence length :

240

specificity :

This assay has high sensitivity and excellent specificity for detection of Human SOD3. No significant cross-reactivity or interference between Human SOD3 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates, saliva, urine. Assay Type: Sandwich. Detection Range: 7.8 pg/ml-500 pg/ml. Sensitivity: 1.95 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SOD3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SOD3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SOD3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SOD3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

118582275

ncbi acc num :

NP_003093.2

ncbi gb acc num :

NM_003102.2

uniprot acc num :

P08294

ncbi mol weight :

25,851 Da

ncbi pathways :

Folate Metabolism Pathway (198833); Oxidative Stress Pathway (198916); Selenium Pathway (198825); Superoxide Radicals Degradation Pathway (142440)

ncbi summary :

This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. The product of this gene is thought to protect the brain, lungs, and other tissues from oxidative stress. The protein is secreted into the extracellular space and forms a glycosylated homotetramer that is anchored to the extracellular matrix (ECM) and cell surfaces through an interaction with heparan sulfate proteoglycan and collagen. A fraction of the protein is cleaved near the C-terminus before secretion to generate circulating tetramers that do not interact with the ECM. [provided by RefSeq, Jul 2008]

uniprot summary :

SOD3: Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. Belongs to the Cu-Zn superoxide dismutase family. Protein type: Oxidoreductase; Secreted; Secreted, signal peptide; EC 1.15.1.1. Chromosomal Location of Human Ortholog: 4p15.2. Cellular Component: extracellular matrix; extracellular space; Golgi lumen; cytoplasm; extracellular region; trans-Golgi network; nucleus. Molecular Function: heparin binding; protein binding; copper ion binding; zinc ion binding; superoxide dismutase activity. Biological Process: response to reactive oxygen species; removal of superoxide radicals; response to copper ion; response to hypoxia

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage