Mouse Protein Wnt-7b, WNT7B ELISA Kit | MyBioSource MBS936824 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Protein Wnt-7b, WNT7B ELISA Kit

catalog :

MBS936824

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS936824

products type :

ELISA Kit

products full name :

Mouse Protein Wnt-7b, WNT7B ELISA Kit

products short name :

wingless-type MMTV integration site family, member 7B

products name syn :

Mouse Protein Wnt-7b (WNT7B) ELISA kit; wingless-type MMTV integration site family; member 7B

other names :

protein Wnt-7b isoform 3; Protein Wnt-7b; protein Wnt-7b; wingless-related MMTV integration site 7B; wingless-type MMTV integration site family, member 7B

products gene name :

WNT7B

other gene names :

Wnt7b; Wnt7b; Wnt-7b; Wnt-7b

uniprot entry name :

WNT7B_MOUSE

reactivity :

Mouse

sequence length :

282

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse WNT7B. No significant cross-reactivity or interference between mouse WNT7B and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: 0.078 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT7B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT7B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT7B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT7B bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

254692924

ncbi acc num :

NP_001157105.1

ncbi gb acc num :

NM_001163633.1

uniprot acc num :

P28047

ncbi mol weight :

39,302 Da

ncbi pathways :

Basal Cell Carcinoma Pathway (83306); Basal Cell Carcinoma Pathway (525); ESC Pluripotency Pathways (198374); HTLV-I Infection Pathway (373903); HTLV-I Infection Pathway (373889); Hedgehog Signaling Pathway (83260); Hedgehog Signaling Pathway (474); Melanogenesis Pathway (83289); Melanogenesis Pathway (504); Pathways In Cancer (83298)

uniprot summary :

WNT7B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family. Protein type: Secreted; Secreted, signal peptide. Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; cytoplasm; plasma membrane; extracellular region. Molecular Function: protein binding; frizzled binding; receptor agonist activity; receptor binding. Biological Process: oxygen homeostasis; cell proliferation in forebrain; multicellular organismal development; positive regulation of JNK cascade; Wnt receptor signaling pathway through beta-catenin; signal transduction; anatomical structure regression; activation of JNK activity; negative regulation of neurogenesis; neuron differentiation; cellular metabolic process; central nervous system vasculogenesis; vasculature development; homeostatic process; positive regulation of cell proliferation; synapse organization and biogenesis; regulation of cell projection size; establishment and/or maintenance of polarity of embryonic epithelium; angiogenesis; neurite development; Wnt receptor signaling pathway; cell fate commitment; in utero embryonic development; odontogenesis of dentine-containing teeth; positive regulation of osteoblast differentiation; stem cell development; negative regulation of smoothened signaling pathway; embryonic organ development; smooth muscle cell differentiation; neurite morphogenesis; lung development

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage