ELISA/assay

Human GTPase KRas, KRAS ELISA Kit

MBS936810

48-Strip-Wells

510 USD

ELISA Kit

Human GTPase KRas, KRAS ELISA Kit

v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Human GTPase KRas (KRAS) ELISA kit; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RASK2; K-ras p21 protein; Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog; PR310 c-K-ras oncogene; c-K-ras2 protein; ; v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

GTPase KRas isoform b; GTPase KRas; GTPase KRas; K-Ras 2; c-Ki-ras; oncogene KRAS2; K-ras p21 protein; c-Kirsten-ras protein; PR310 c-K-ras oncogene; transforming protein p21; cellular c-Ki-ras2 proto-oncogene; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog; Kirsten rat sarcoma viral oncogene homolog; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-rasCleaved into the following chain:GTPase KRas, N-terminally processed

KRAS

KRAS; KRAS; NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS2; RASK2

RASK_HUMAN

Human

188

This assay has high sensitivity and excellent specificity for detection of human KRAS. No significant cross-reactivity or interference between human KRAS and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 23.5 pg/ml -1500 pg/ml. Sensitivity: 5.8 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KRAS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KRAS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRAS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRAS bound in the initial step. The color development is stopped and the intensity of the color is measured.

15718761

NP_004976.2

NM_004985.4

P01116

21,656 Da

ARMS-mediated Activation Pathway (106466); Activation Of RAS In B Cells Pathway (576252); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adaptive Immune System Pathway (366160); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Axon Guidance Pathway (83065)

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]

KRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts with PHLPP. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: G protein; G protein, monomeric; Motility/polarity/chemotaxis; Oncoprotein; G protein, monomeric, Ras. Chromosomal Location of Human Ortholog: 12p12.1. Cellular Component: extrinsic to internal side of plasma membrane; focal adhesion; mitochondrion; membrane; cytoplasm; plasma membrane; cytosol; lipid raft. Molecular Function: protein binding; GDP binding; GTP binding; GMP binding; protein complex binding; LRR domain binding. Biological Process: regulation of long-term neuronal synaptic plasticity; axon guidance; activation of MAPKK activity; nerve growth factor receptor signaling pathway; response to glucocorticoid stimulus; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; small GTPase mediated signal transduction; positive regulation of cell proliferation; visual learning; negative regulation of neuron apoptosis; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; positive regulation of nitric-oxide synthase activity; MAPKKK cascade; response to mineralocorticoid stimulus; social behavior; regulation of synaptic transmission, GABAergic; positive regulation of Rac protein signal transduction; negative regulation of cell differentiation; Ras protein signal transduction; insulin receptor signaling pathway; innate immune response; striated muscle cell differentiation; positive regulation of protein amino acid phosphorylation; actin cytoskeleton organization and biogenesis; vascular endothelial growth factor receptor signaling pathway; blood coagulation; leukocyte migration. Disease: Bladder Cancer; Gastric Cancer, Hereditary Diffuse; Pancreatic Cancer; Noonan Syndrome 3; Leukemia, Acute Myeloid; Lung Cancer; Breast Cancer; Schimmelpenning-feuerstein-mims Syndrome; Cardiofaciocutaneous Syndrome 2

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800