catalog number :
MBS936549
products type :
ELISA Kit
products full name :
Human Alkaline phosphatase, placental-like, ALPPL2 ELISA Kit
products short name :
alkaline phosphatase, placental-like 2
products name syn :
Human Alkaline phosphatase; placental-like (ALPPL2) ELISA kit; ALPG; ALPPL; GCAP; Nagao isozyme; germ cell alkaline phosphatase; placental-like alkaline phosphatase; testicular and thymus alkaline phosphatase; alkaline phosphatase; placental-like 2
other names :
alkaline phosphatase, placental-like preproprotein; Alkaline phosphatase, placental-like; alkaline phosphatase, placental-like; ALP-1; Nagao isozyme; germ cell alkaline phosphatase; alkaline phosphatase Nagao isozyme; placental-like alkaline phosphatase; testicular and thymus alkaline phosphatase; alkaline phosphatase, placental-like 2; ALP-1; Alkaline phosphatase Nagao isozyme; Germ cell alkaline phosphatase; GCAP; Placental alkaline phosphatase-like; PLAP-like
products gene name :
ALPPL2
other gene names :
ALPPL2; ALPPL2; ALPG; GCAP; ALPPL; ALPPL; GCAP; PLAP-like
uniprot entry name :
PPBN_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Human ALPPL2. No significant cross-reactivity or interference between Human ALPPL2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates, cell lysates. Detection Range: 1.25 ng/ml-80 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.31 ng/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ALPPL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ALPPL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALPPL2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALPPL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_112603.2
ncbi gb acc num :
NM_031313.2
ncbi mol weight :
57,377 Da
ncbi pathways :
Folate Biosynthesis Pathway 83018!!Folate Biosynthesis Pathway 404
ncbi summary :
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme, localized to testis, thymus and certain germ cell tumors, that is closely related to both the placental and intestinal forms of alkaline phosphatase. [provided by RefSeq, Jul 2008]