ELISA/assay

Mouse Receptor-interacting serine/threonine-protein kinase 1, RIPK1 ELISA Kit

MBS936456

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Mouse Receptor-interacting serine/threonine-protein kinase 1, RIPK1 ELISA Kit

[receptor (TNFRSF) -interacting serine-threonine kinase 1]

[Mouse Receptor-interacting serine/threonine-protein kinase 1 (RIPK1) ELISA kit; FLJ39204; RIP; RIP1; OTTHUMP00000015955; cell death protein RIP; receptor interacting protein; serine/threonine-protein kinase RIP; receptor (TNFRSF) -interacting serine-threonine kinase 1]

[receptor-interacting serine/threonine-protein kinase 1; Receptor-interacting serine/threonine-protein kinase 1; receptor-interacting serine/threonine-protein kinase 1; RIP-1; cell death protein RIP; receptor-interacting protein 1; serine/threonine-protein kinase RIP; receptor (TNFRSF)-interacting serine-threonine kinase 1; Cell death protein RIP; Receptor-interacting protein 1; RIP-1; Serine/threonine-protein kinase RIP]

[RIPK1]

[Ripk1; Ripk1; RIP; Rinp; Rip1; D330015H01Rik; Rinp; Rip; RIP-1]

RIPK1_MOUSE

Mouse

656

This assay has high sensitivity and excellent specificity for detection of mouse RIPK1. No significant cross-reactivity or interference between mouse RIPK1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Quantitative Sandwich. Detection Range: 15.6 ng/ml -1000 ng/ml. Sensitivity: 3.9 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RIPK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RIPK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RIPK1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RIPK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

34328467

NP_033094.3

NM_009068.3

Q60855

74,854 Da

Activation Of Pro-Caspase 8 Pathway (574593); Apoptosis Pathway (198339); Apoptosis Pathway (83257); Apoptosis Pathway (470); Apoptosis Pathway (574586); Apoptosis Modulation By HSP70 Pathway (198355); Apoptosis Signaling Pathway (522973); Caspase-8 Is Formed From Procaspase-8 Pathway (574592); Cytosolic DNA-sensing Pathway (125146); Cytosolic DNA-sensing Pathway (124833)

RIPK1: Serine-threonine kinase which transduces inflammatory and cell-death signals (necroptosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Ubiquitination by TRAF2 via Lys-63 -link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necroptosis-inducing complex. Interacts (via RIP homotypic interaction motif) with RIPK3 (via RIP homotypic interaction motif); this interaction induces RIPK1 necroptosis-specific phosphorylation, formation of the necroptosis-inducing complex. Interacts (via the death domain) with TNFRSF6 (via the death domain) and TRADD (via the death domain). Is recruited by TRADD to TNFRSF1A in a TNF-dependent process. Binds RNF216, EGFR, IKBKG, TRAF1, TRAF2 and TRAF3. Interacts with BNLF1. Interacts with SQSTM1 upon TNF-alpha stimulation. May interact with MAVS/IPS1. Interacts with ZFAND5. Interacts with RBCK1. Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2 and XIAP/BIRC4. Inhibited by necrostatin-1. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Protein kinase, TKL; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); TKL group; RIPK family. Cellular Component: protein complex; mitochondrion; membrane; cytoplasm; plasma membrane; receptor complex; lipid raft. Molecular Function: transferase activity; identical protein binding; protein serine/threonine kinase activity; protein binding; ubiquitin protein ligase binding; death receptor binding; transferase activity, transferring phosphorus-containing groups; protein complex binding; nucleotide binding; kinase activity; ATP binding; protein kinase activity. Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; positive regulation of apoptosis; protein heterooligomerization; apoptosis; protein amino acid autophosphorylation; programmed cell death; positive regulation of JNK cascade; negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of transferase activity; signal transduction; positive regulation of tumor necrosis factor production; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; positive regulation of programmed cell death; positive regulation of MAPKKK cascade; cellular protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; positive regulation of macrophage differentiation; phosphorylation; positive regulation of phosphorylation; protein homooligomerization

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

495

96-Strip-Wells

710

5x96-Strip-Wells

2705

10x96-Strip-Wells

5145