ELISA/assay

Human Kelch-like ECH-associated protein 1, KEAP1 ELISA Kit

MBS936418

48-Strip-Wells

510 USD

ELISA Kit

Human Kelch-like ECH-associated protein 1, KEAP1 ELISA Kit

kelch-like ECH-associated protein 1

Human Kelch-like ECH-associated protein 1 (KEAP1) ELISA kit; INrf2; KIAA0132; KLHL19; MGC10630; MGC1114; MGC20887; MGC4407; MGC9454; cytosolic inhibitor of Nrf2; kelch-like ECH-associated protein 1

kelch-like ECH-associated protein 1; Kelch-like ECH-associated protein 1; kelch-like ECH-associated protein 1; kelch-like protein 19; cytosolic inhibitor of Nrf2; kelch-like family member 19; kelch-like ECH-associated protein 1; Cytosolic inhibitor of Nrf2; INrf2; Kelch-like protein 19

KEAP1

KEAP1; KEAP1; INrf2; KLHL19; INRF2; KIAA0132; KLHL19; INrf2

KEAP1_HUMAN

Human

624

This assay has high sensitivity and excellent specificity for detection of human KEAP1. No significant cross-reactivity or interference between human KEAP1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 25 pg/ml -1600 pg/ml. Sensitivity: 6.25 pg /ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KEAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KEAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KEAP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KEAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

22027642

NP_036421.2

NM_012289.3

Q14145

69,666 Da

Adaptive Immune System Pathway (366160); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Immune System Pathway (106386); Keap1-Nrf2 Pathway (198777); Ubiquitin Mediated Proteolysis Pathway (83056); Ubiquitin Mediated Proteolysis Pathway (466)

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]

KEAP1: a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Has tumor suppressor activity. Retains NRF2, BPTF and PGAM5 in the cytosol. Targets NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and of antioxidant response element-mediated detoxifying enzyme gene expression. Somatic mutations of KEAP1 or NRF2 commonly occur in solid cancers, irrespective of histological type, resulting in the constitutive transcription of cytoprotective genes. Genetic disruption of the KEAP1/CUL3 E3 ubiquitin ligase complex leads to the activation of the NF-kappaB pathway in lung cancer. Alternative activation of the cytoprotective NRF2 pathway exist in the absence of mutations in KEAP1 and HRF2: WTX, PALB2, SQSTM1, and DPP3 can competitively bind KEAP1. These proteins and others that bind KEAP1 contain an ¿ETGE¿ amino acid motif, which matches the KEAP1 interaction motif found in NRF2. Binding of these ¿ETGE¿-containing proteins can displace NRF2, freeing it to translocate to the nucleus and implement its cytoprotective transcriptional pathway. The DPP3 gene is frequently overexpressed in squamous cell lung carcinomas with high wt NRF2 activity. Its expression level significantly correlates with the response rate and progression-free survival of platinum-based first-line chemotherapy. May be a useful biomarker for predicting the chemosensitivity of patients with advanced-stage NSCLC. Broadly expressed, with highest levels in skeletal muscle. Ubiquitination and subsequent degradation of PGAM5 is inhibited by oxidative stress and sulforaphane. Protein type: Endoplasmic reticulum; Transcription regulation; Motility/polarity/chemotaxis; Ubiquitin conjugating system. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: nucleoplasm; endoplasmic reticulum; cytoplasm; microtubule organizing center; midbody; cytosol. Molecular Function: protein binding; transcription factor binding. Biological Process: transcription, DNA-dependent; in utero embryonic development; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of transcription factor activity; proteasomal ubiquitin-independent protein catabolic process; protein ubiquitination; regulation of epidermal cell differentiation

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800