catalog number :
MBS936332
products type :
ELISA Kit
products full name :
Human Beta-1 adrenergic receptor, ADRB1 ELISA Kit
products short name :
adrenergic, beta-1-, receptor
products name syn :
Human Beta-1 adrenergic receptor (ADRB1) ELISA kit; ADRB1R; B1AR; BETA1AR; RHR; beta-1-adrenergic receptor; adrenergic; beta-1-; receptor
other names :
beta-1 adrenergic receptor; Beta-1 adrenergic receptor; beta-1 adrenergic receptor; beta-1 adrenoceptor; beta-1 adrenoreceptor; beta 1 adrenergic receptor; adrenergic, beta-1-, receptor; Beta-1 adrenoreceptor; Beta-1 adrenoceptor
products gene name :
ADRB1
other gene names :
ADRB1; ADRB1; Beta-1 adrenoceptor
uniprot entry name :
ADRB1_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of human ADRB1. No significant cross-reactivity or interference between human ADRB1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 25 pg/ml -1600 pg/ml. Sensitivity: The minimum detectable dose of human ADRB1 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADRB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADRB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADRB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADRB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_919242.1
ncbi gb acc num :
NM_194266.1
ncbi mol weight :
50,137 Da
ncbi pathways :
Adrenoceptors Pathway (581444); Amine Ligand-binding Receptors Pathway (581442); Calcium Regulation In The Cardiac Cell Pathway (198579); Calcium Signaling Pathway (84289); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (581436); Dilated Cardiomyopathy Pathway (121480); Dilated Cardiomyopathy Pathway (121285); Endocytosis Pathway (102272); Endocytosis Pathway (102181)
uniprot summary :
Function: Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Mediates Ras activation through G(s)-alpha- and cAMP-mediated signaling
size4 :
10x96-Strip-Wells