catalog number :
MBS936317
products type :
ELISA Kit
products full name :
Mouse Angiopoietin-related protein 6, ANGPTL6 ELISA Kit
products short name :
angiopoietin-like 6
products name syn :
Mouse Angiopoietin-related protein 6 (ANGPTL6) ELISA kit; AGF; ARP5; angiopoietin-related protein 5; angiopoietin-like 6
other names :
angiopoietin-related protein 6; Angiopoietin-related protein 6; angiopoietin-related protein 6; angiopoietin-like protein 6; angiopoietin-related protein 5; Angiopoietin-related growth factor; angiopoietin-like 6; Angiopoietin-like protein 6; Angiopoietin-related growth factor
products gene name :
ANGPTL6
other gene names :
Angptl6; Angptl6; AGF; ARP3; Arp5; 6330404E11Rik; Agf
uniprot entry name :
ANGL6_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse ANGPTL6. No significant cross-reactivity or interference between mouse ANGPTL6 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml -4000 pg/ml. Sensitivity: The minimum detectable dose of mouse ANGPTL6 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANGPTL6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANGPTL6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANGPTL6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANGPTL6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_660136.1
ncbi gb acc num :
NM_145154.2
ncbi mol weight :
51,095 Da
uniprot summary :
ANGPTL6: May play a role in the wound healing process. May promote epidermal proliferation, remodeling and regeneration. May promote the chemotactic activity of endothelial cells and induce neovascularization. May counteract high-fat diet-induced obesity and related insulin resistance through increased energy expenditure. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular region; secretory granule. Biological Process: multicellular organismal development; angiogenesis; cell differentiation
size4 :
10x96-Strip-Wells