Human Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2, PLOD2 ELISA Kit | MyBioSource MBS936181 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2, PLOD2 ELISA Kit

catalog :

MBS936181

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS936181

products type :

ELISA Kit

products full name :

Human Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2, PLOD2 ELISA Kit

products short name :

procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2

products name syn :

Human Procollagen-lysine; 2-oxoglutarate 5-dioxygenase 2 (PLOD2) ELISA kit; LH2; TLH; lysine hydroxylase 2; lysyl hydroxylase 2; telopeptide lysyl hydroxylase; procollagen-lysine; 2-oxoglutarate 5-dioxygenase 2

other names :

procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 isoform 2; Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2; procollagen-lysine,2-oxoglutarate 5-dioxygenase 2; lysyl hydroxlase 2; lysyl hydroxylase 2; lysine hydroxylase 2; telopeptide lysyl hydroxylase; procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2; Lysyl hydroxylase 2; LH2

products gene name :

PLOD2

other gene names :

PLOD2; PLOD2; LH2; TLH; LH2

uniprot entry name :

PLOD2_HUMAN

reactivity :

Human

sequence length :

737

specificity :

This assay has high sensitivity and excellent specificity for detection of human PLOD2. No significant cross-reactivity or interference between human PLOD2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 9.375 pg/ml -600 pg/ml. Sensitivity: The minimum detectable dose of human PLOD2 is typically less than 2.34 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLOD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLOD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLOD2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLOD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

62739166

ncbi acc num :

NP_000926.2

ncbi gb acc num :

NM_000935.2

uniprot acc num :

O00469

ncbi mol weight :

84,686 Da

ncbi pathways :

Lysine Degradation Pathway (82956); Lysine Degradation Pathway (320)

ncbi summary :

The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

uniprot summary :

PLOD2: Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. Defects in PLOD2 are the cause of Bruck syndrome type 2 (BRKS2). Bruck syndrome, also known as osteogenesis imperfecta with congenital joint contractures, is an autosomal recessive disease characterized by generalized osteopenia, joint contractures at birth, fragile bones and short stature. It can be distinguished from osteogenesis imperfecta by the absence of hearing loss and dentinogenesis imperfecta, and by the presence of clubfoot and congenital joint limitations. The molecular defect is an aberrant cross-linking of bone collagen, due to underhydroxylation of lysine residues within the telopeptides of type I collagen, whereas the lysine residues in the triple helix are normal. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Oxidoreductase; EC 1.14.11.4; Amino Acid Metabolism - lysine degradation; Endoplasmic reticulum. Chromosomal Location of Human Ortholog: 3q24. Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; rough endoplasmic reticulum membrane. Molecular Function: L-ascorbic acid binding; iron ion binding; procollagen-lysine 5-dioxygenase activity. Biological Process: extracellular matrix organization and biogenesis; response to hypoxia; protein modification process. Disease: Bruck Syndrome 2

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage