ELISA/assay

Human Interferon kappa, IFNK ELISA Kit

MBS936153

48-Strip-Wells

510 USD

ELISA Kit

Human Interferon kappa, IFNK ELISA Kit

interferon, kappa

Human Interferon kappa (IFNK) ELISA kit; RP11-27J8.1; interferon kappa; interferon-like protein; interferon; kappa

interferon kappa; Interferon kappa; interferon kappa; IFN-kappa; interferon-like protein; interferon, kappa

IFNK

IFNK; IFNK; IFNT1; INFE1; UNQ6124/PRO20084; IFN-kappa

IFNK_HUMAN

Human

207

This assay has high sensitivity and excellent specificity for detection of human IFNK. No significant cross-reactivity or interference between human IFNK and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: Typically less than 3.9 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IFNK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFNK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFNK is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFNK bound in the initial step. The color development is stopped and the intensity of the color is measured.

112789564

NP_064509.2

NM_020124.2

Q9P0W0

25,218 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); RIG-I-like Receptor Signaling Pathway (117292); RIG-I-like Receptor Signaling Pathway (116128)

This gene encodes a member of the type I interferon family. Type I interferons are a group of related glycoproteins that play an important role in host defenses against viral infections. This protein is expressed in keratinocytes and the gene is found on chromosome 9, adjacent to the type I interferon cluster. [provided by RefSeq, Jul 2008]

IFNK: May play a role in the regulation of immune cell function. Cytokine that imparts cellular protection against viral infection in a species-specific manner. Activates the interferon- stimulated response element signaling pathway. It is able to directly modulate cytokine release from monocytes and dendritic cells. Binds heparin. Belongs to the alpha/beta interferon family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: -. Cellular Component: extracellular space; extracellular region. Molecular Function: interferon-alpha/beta receptor binding; cytokine activity. Biological Process: B cell proliferation; adaptive immune response; cytokine and chemokine mediated signaling pathway; response to virus; regulation of MHC class I biosynthetic process; natural killer cell activation; humoral immune response; T cell activation during immune response; negative regulation of cell proliferation; response to exogenous dsRNA; regulation of transcription, DNA-dependent; B cell differentiation; positive regulation of innate immune response; natural killer cell activation during immune response; innate immune response; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800