Human Fibulin-1, FBLN1 ELISA Kit | MyBioSource MBS936048 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Fibulin-1, FBLN1 ELISA Kit

catalog :

MBS936048

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS936048

products type :

ELISA Kit

products full name :

Human Fibulin-1, FBLN1 ELISA Kit

products short name :

fibulin 1

products name syn :

Human Fibulin-1 (FBLN1) ELISA kit; CTA-941F9.7; FBLN; ; fibulin 1

other names :

fibulin-1 isoform C; Fibulin-1; fibulin-1; fibulin 1

products gene name :

FBLN1

other gene names :

FBLN1; FBLN1; FBLN; FIBL1; PP213; FIBL-1

uniprot entry name :

FBLN1_HUMAN

reactivity :

Human

sequence length :

683

specificity :

This assay has high sensitivity and excellent specificity for detection of Human FBLN1. No significant cross-reactivity or interference between Human FBLN1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 6.25 g/ml-400 g/ml. Sensitivity: 1.56 g/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Host-virus interaction

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for fibulin-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any fibulin-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for fibulin-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of fibulin-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

34734062

ncbi acc num :

NP_001987.2

ncbi gb acc num :

NM_001996.3

uniprot acc num :

P23142

ncbi mol weight :

77,214 Da

ncbi summary :

Fibulin 1 is a secreted glycoprotein that becomes incorporated into a fibrillar extracellular matrix. Calcium-binding is apparently required to mediate its binding to laminin and nidogen. It mediates platelet adhesion via binding fibrinogen. Four splice variants which differ in the 3' end have been identified. Each variant encodes a different isoform, but no functional distinctions have been identified among the four variants. [provided by RefSeq, Jul 2008]

uniprot summary :

FBLN1: Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP. A chromosomal aberration involving FBLN1 is found in a complex type of synpolydactyly referred to as 3/3-prime/4 synpolydactyly associated with metacarpal and metatarsal synostoses. Reciprocal translocation t(12;22)(p11.2;q13.3) with C12orf2. Fibroblasts derived from a patient with synpolydactyly displayed alterations in the level of isoform D splice variant incorporated into the ECM and secreted into the conditioned culture medium. By contrast, the expression of isoform C was not perturbed in the patients fibroblasts. Furthermore, no aberrant polypeptides were detected in extracts of cultured patients fibroblasts. The translocation t(12;22) may result in haploinsufficiency of the isoform D splice variant, which could lead to the observed limb malformation. Elevated expression and altered processing of FBLN1 protein is associated with human breast cancer. Belongs to the fibulin family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Extracellular matrix; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 22q13.31. Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region; basement membrane. Molecular Function: protease activator activity; extracellular matrix structural constituent; calcium ion binding. Biological Process: extracellular matrix organization and biogenesis; viral reproduction; embryo implantation. Disease: Synpolydactyly 2

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage