ELISA/assay

Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 3, ENPP3 ELISA Kit

MBS935980

48-Strip-Wells

510 USD

ELISA Kit

Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 3, ENPP3 ELISA Kit

ectonucleotide pyrophosphatase/phosphodiesterase 3

Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 (ENPP3) ELISA kit; RP5-988G15.3; B10; CD203c; NPP3; PD-IBETA; PDNP3; OTTHUMP00000017901; dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) ; dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) ; gp13; ectonucleotide pyrophosphatase/phosphodiesterase 3

ectonucleotide pyrophosphatase/phosphodiesterase family member 3; Ectonucleotide pyrophosphatase/phosphodiesterase family member 3; ectonucleotide pyrophosphatase/phosphodiesterase family member 3; gp130RB13-6; phosphodiesterase-I beta; phosphodiesterase I/nucleotide pyrophosphatase 3; dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3); dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3); ectonucleotide pyrophosphatase/phosphodiesterase 3; Phosphodiesterase I beta; PD-Ibeta; Phosphodiesterase I/nucleotide pyrophosphatase 3; CD_antigen: CD203cIncluding the following 2 domains:Alkaline phosphodiesterase I (EC:3.1.4.1); Nucleotide pyrophosphatase (EC:3.6.1.9); NPPase

ENPP3

ENPP3; ENPP3; B10; NPP3; PDNP3; CD203c; PD-IBETA; PDNP3; E-NPP 3; PD-Ibeta; NPPase

ENPP3_HUMAN

Human

875

This assay has high sensitivity and excellent specificity for detection of human ENPP3. No significant cross-reactivity or interference between human ENPP3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell lysates. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: The minimum detectable dose of human ENPP3 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Assay Type: Sandwich

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ENPP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ENPP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ENPP3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ENPP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

111160296

NP_005012.2

NM_005021.3

O14638

100,124 Da

Nicotinate And Nicotinamide Metabolism Pathway (83014); Nicotinate And Nicotinamide Metabolism Pathway (400); Pantothenate And CoA Biosynthesis Pathway (83015); Pantothenate And CoA Biosynthesis Pathway (401); Purine Metabolism Pathway (82944); Purine Metabolism Pathway (307); Riboflavin Metabolism Pathway (83012); Riboflavin Metabolism Pathway (398); Starch And Sucrose Metabolism Pathway (82974); Starch And Sucrose Metabolism Pathway (344)

The protein encoded by this gene belongs to a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides. These ectoenzymes possess ATPase and ATP pyrophosphatase activities and are type II transmembrane proteins. Expression of the related rat mRNA has been found in a subset of immature glial cells and in the alimentary tract. The corresponding rat protein has been detected in the pancreas, small intestine, colon, and liver. The human mRNA is expressed in glioma cells, prostate, and uterus. Expression of the human protein has been detected in uterus, basophils, and mast cells. [provided by RefSeq, Jul 2008]

ENPP3: Cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. Protein type: Cofactor and Vitamin Metabolism - riboflavin; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Membrane protein, integral; Nucleotide Metabolism - purine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Motility/polarity/chemotaxis; EC 3.6.1.9; EC 3.1.4.1; Phosphodiesterase; Carbohydrate Metabolism - starch and sucrose. Chromosomal Location of Human Ortholog: 6q22. Cellular Component: integral to plasma membrane; perinuclear region of cytoplasm. Molecular Function: nucleotide diphosphatase activity; phosphodiesterase I activity; nucleic acid binding; metal ion binding; nucleoside-triphosphate diphosphatase activity; scavenger receptor activity; polysaccharide binding. Biological Process: receptor-mediated endocytosis; nucleoside triphosphate catabolic process; immune response; phosphate metabolic process; inorganic diphosphate transport

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800