ELISA/assay

Mouse Eukaryotic Translation Initiation Factor 4E (EIF4E) ELISA Kit

MBS9358675

48-Strip-Wells

470 USD

ELISA Kit

Mouse Eukaryotic Translation Initiation Factor 4E (EIF4E) ELISA Kit

[Eukaryotic Translation Initiation Factor 4E (EIF4E)]

[eukaryotic translation initiation factor 4E; Eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein]

[EIF4E]

[Eif4e; Eif4e; eIF-4E; eIF4E]

IF4E_RAT

Mouse

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Samples: Body fluids, tissue homogenates, secretions or feces samples. Assay Type: Quantitative Sandwich. Detection Range: 62.5 pg/ml - 2000 pg/ml. Sensitivity: 10 pg/ml.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of EIF4E (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

16758870

NP_446426.1

NM_053974.2

25,053 Da

Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway (1333746); Alpha6-Beta4 Integrin Signaling Pathway (198503); Antiviral Mechanism By IFN-stimulated Genes Pathway (1332890); Cap-dependent Translation Initiation Pathway (1333743); Cytokine Signaling In Immune System Pathway (1332884); Deadenylation Of MRNA Pathway (1333725); Deadenylation-dependent MRNA Decay Pathway (1333724); Eukaryotic Translation Initiation Pathway (1333742); GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway (1333749); Gene Expression Pathway (1333665)

binds to the mRNA 7-methylguanosine cap and mediates mRNA binding to the 40S ribosome in the rate limiting step in translational initiation [RGD, Feb 2006]

EIF4E: a protein of the eukaryotic initiation factor 4E family. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the EIF4F complex. Protein type: Translation initiation; Translation. Cellular Component: cytoplasm; eukaryotic translation initiation factor 4F complex; mRNA cap complex; perinuclear region of cytoplasm; protein complex; stress granule. Molecular Function: enzyme binding; eukaryotic initiation factor 4G binding; protein binding; translation initiation factor activity. Biological Process: behavioral fear response; G1/S transition of mitotic cell cycle; lung development; negative regulation of neuron differentiation; negative regulation of translation; positive regulation of mitotic cell cycle; regulation of translation; stem cell maintenance; translational initiation

48-Strip-Wells

470 USD

96-Strip-Wells

680

5x96-Strip-Wells

3100

10x96-Strip-Wells

6095