ELISA/assay

Human Peptidyl-prolyl cis-trans isomerase FKBP1A, FKBP1A ELISA Kit

MBS935796

48-Strip-Wells

510 USD

ELISA Kit

Human Peptidyl-prolyl cis-trans isomerase FKBP1A, FKBP1A ELISA Kit

FK506 binding protein 1A, 12kDa

Human Peptidyl-prolyl cis-trans isomerase FKBP1A (FKBP1A) ELISA kit; RP11-314N13.2; FKBP-12; FKBP1; FKBP12; FKBP12C; PKC12; PKCI2; PPIASE; FK506 binding protein12; FK506-binding protein 1; FK506-binding protein 12; FK506-binding protein 1A (12kD) ; FK506-binding protein; ; FK506 binding protein 1A; 12kDa

peptidyl-prolyl cis-trans isomerase FKBP1A isoform a; Peptidyl-prolyl cis-trans isomerase FKBP1A; peptidyl-prolyl cis-trans isomerase FKBP1A; rotamase; 12 kDa FKBP; calstabin 1; calstabin-1; FKBP12-Exip3; PPIase FKBP1A; immunophilin FKBP12; FK506 binding protein12; FK506-binding protein 1; FK506-binding protein 12; 12 kDa FK506-binding protein; protein kinase C inhibitor 2; FK506-binding protein 1A (12kD); FK506-binding protein, T-cell, 12-kD; FK506 binding protein 1A, 12kDa; 12 kDa FK506-binding protein; 12 kDa FKBP; FKBP-12; Calstabin-1; FK506-binding protein 1A; FKBP-1A; Immunophilin FKBP12; Rotamase

FKBP1A

FKBP1A; FKBP1A; FKBP1; PKC12; PKCI2; FKBP12; PPIASE; FKBP-12; FKBP-1A; FKBP1; FKBP12; PPIase FKBP1A; 12 kDa FKBP; FKBP-12; FKBP-1A

FKB1A_HUMAN

Human

108

This assay has high sensitivity and excellent specificity for detection of Human FKBP1A. No significant cross-reactivity or interference between Human FKBP1A and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: 7.81 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FKBP1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FKBP1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FKBP1A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FKBP1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

4503725

NP_000792.1

NM_000801.4

P62942

11,951 Da

ALK1 Pathway (137985); ALK1 Signaling Events Pathway (137968); ALK2 Signaling Events Pathway (137983); Alpha-synuclein Signaling Pathway (137913); Calcium Regulation In The Cardiac Cell Pathway (198906); Calcium Signaling In The CD4+ TCR Pathway (137941); Role Of Calcineurin-dependent NFAT Signaling In Lymphocytes Pathway (137953); TGF Beta Signaling Pathway (198810); TGF-beta Receptor Signaling Pathway (198774); TGF-beta Receptor Signaling Pathway (138024)

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium. Multiple alternatively spliced variants, encoding the same protein, have been identified. The human genome contains five pseudogenes related to this gene, at least one of which is transcribed. [provided by RefSeq, Sep 2008]

FKBP12: an immunophilin and peptidyl-prolyl cis-trans isomerase that binds to the immunosuppressant drugs FK506 (a.k.a. tacrolimus or Fujimycin) and rapamycin (a.k.a. Sirolimus). The FKBP12/rapamycin complex binds NFAT transcription factors and inhibits the induction of T cell genes including IL-2, IL-3, IL-4, TNF-alpha and GM-CSF. FK506 is used in treating patients after organ transplant and those suffering from autoimmune disorders. The immunosuppressant activity of FKBP12 is not apparently related to its prolyl isomerase activity. The FKBP12/rapamycin complex inhibits the mammalian target of rapamycin (mTOR) pathway by directly binding the mTOR Complex1 (mTORC1). mTORC1 contains Raptor, mLST8, and PRAS40, and interacts with DEPTOR, which inhibits its activity. mTOR, a S/T protein kinase, is a central regulator of cellular growth and metabolism. Its inhibition enhances the dependence on aerobic glycolysis in leukemic cells. Protein type: EC 5.2.1.8; Isomerase. Chromosomal Location of Human Ortholog: 20p13. Cellular Component: endoplasmic reticulum membrane; sarcoplasmic reticulum membrane; membrane; cytoplasm; nerve terminal; Z disc; cytosol. Molecular Function: protein binding; signal transducer activity; FK506 binding; enzyme binding; protein homodimerization activity; peptidyl-prolyl cis-trans isomerase activity; activin binding; macrolide binding; Hsp70 protein binding; calcium channel inhibitor activity; SMAD binding; transforming growth factor beta receptor binding. Biological Process: heart morphogenesis; protein maturation via protein folding; protein peptidyl-prolyl isomerization; protein folding; positive regulation of protein binding; T cell activation; response to caffeine; T cell proliferation; regulation of protein localization; negative regulation of protein amino acid phosphorylation; muscle contraction; negative regulation of release of sequestered calcium ion into cytosol; transforming growth factor beta receptor signaling pathway; fibril organization and biogenesis; ventricular cardiac muscle morphogenesis; protein refolding; response to iron ion; regulation of immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; cytokine and chemokine mediated signaling pathway; regulation of activin receptor signaling pathway; SMAD protein complex assembly; negative regulation of protein phosphatase type 2B activity; de novo protein folding; positive regulation of protein ubiquitination; release of sequestered calcium ion into cytosol

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800