catalog number :
MBS935746
products type :
ELISA Kit
products full name :
Mouse Protein Wnt-16, WNT16 ELISA Kit
products short name :
wingless-type MMTV integration site family, member 16
products name syn :
Mouse Protein Wnt-16 (WNT16) ELISA kit; wingless-type MMTV integration site family; member 16
other names :
protein Wnt-16; Protein Wnt-16; protein Wnt-16; wingless-related MMTV integration site 16; wingless-type MMTV integration site family, member 16
products gene name :
WNT16
other gene names :
Wnt16; Wnt16; E130309I19Rik
uniprot entry name :
WNT16_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse WNT16. No significant cross-reactivity or interference between mouse WNT16 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml -4000 pg/ml. Sensitivity: The minimum detectable dose of mouse WNT16 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT16 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT16 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_444346.3
ncbi gb acc num :
NM_053116.4
ncbi mol weight :
40,727 Da
ncbi pathways :
Basal Cell Carcinoma Pathway (83306); Basal Cell Carcinoma Pathway (525); ESC Pluripotency Pathways (198374); HTLV-I Infection Pathway (373903); HTLV-I Infection Pathway (373889); Hedgehog Signaling Pathway (83260); Hedgehog Signaling Pathway (474); Melanogenesis Pathway (83289); Melanogenesis Pathway (504); Pathways In Cancer (83298)
uniprot summary :
WNT16: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular space; proteinaceous extracellular matrix; cytoplasm; extracellular region. Molecular Function: frizzled binding; receptor binding. Biological Process: positive regulation of phosphoinositide 3-kinase cascade; keratinocyte differentiation; neuron differentiation; Wnt receptor signaling pathway; cell fate commitment; multicellular organismal development; positive regulation of JNK cascade; keratinocyte proliferation; bone remodeling
size4 :
10x96-Strip-Wells