Human alpha Defensin-4, NP-4 ELISA Kit | MyBioSource MBS935723 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human alpha Defensin-4, NP-4 ELISA Kit

catalog :

MBS935723

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS935723

products type :

ELISA Kit

products full name :

Human alpha Defensin-4, NP-4 ELISA Kit

products short name :

defensin, alpha 4, corticostatin

products name syn :

Human alpha Defensin-4 (NP-4) ELISA Kit; DEF4; HNP-4; HP-4; HP4; MGC120099; MGC138296; corticostatin; defensin; alpha 4; defensin; alpha 4; preproprotein; defensin; alpha 4; corticostatin

other names :

neutrophil defensin 4 preproprotein; Neutrophil defensin 4; neutrophil defensin 4; corticostatin; defensin, alpha 4, preproprotein; defensin, alpha 4, corticostatin; Defensin, alpha 4; HNP-4; HP-4

products gene name :

DEFA4

other gene names :

DEFA4; DEFA4; HP4; DEF4; HP-4; HNP-4; DEF4; HP-4

uniprot entry name :

DEF4_HUMAN

reactivity :

Human

sequence length :

specificity :

This assay has high sensitivity and excellent specificity for detection of human NP-4. No significant cross-reactivity or interference between human NP-4 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of human NP-4 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NP-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NP-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NP-4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NP-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4503303

ncbi acc num :

NP_001916.1

ncbi gb acc num :

NM_001925.1

uniprot acc num :

P12838

ncbi mol weight :

10,504 Da

ncbi pathways :

Alpha-defensins Pathway (530758); Defensins Pathway (530757); Immune System Pathway (106386); Innate Immune System Pathway (106387)

ncbi summary :

Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several alpha defensin genes are clustered on chromosome 8. This gene differs from other genes of this family by an extra 83-base segment that is apparently the result of a recent duplication within the coding region. The protein encoded by this gene, defensin, alpha 4, is found in the neutrophils; it exhibits corticostatic activity and inhibits corticotropin stimulated corticosterone production. [provided by RefSeq, Jul 2008]

uniprot summary :

DEFA4: Has antimicrobial activity against Gram-negative bacteria, and to a lesser extent also against Gram-positive bacteria and fungi. Protects blood cells against infection with HIV-1 (in vitro). Inhibits corticotropin (ACTH)-stimulated corticosterone production. Belongs to the alpha-defensin family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 8p23. Cellular Component: extracellular space; Golgi lumen; extracellular region. Biological Process: killing of cells of another organism; antibacterial humoral response; innate immune response; defense response to fungus

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage