catalog number :
MBS935646
products type :
ELISA Kit
products full name :
Mouse Arginase-1, ARG1 ELISA Kit
products short name :
arginase, liver
products name syn :
Mouse Arginase-1 (ARG1) ELISA kit; arginase 1; liver-type arginase; type I arginase; arginase; liver
other names :
arginase-1; Arginase-1; arginase-1; arginase I; type I arginase; arginase 1, liver; liver-type arginase; arginase, liver; Liver-type arginase; Type I arginase
products gene name :
ARG1
other gene names :
Arg1; Arg1; AI; PGIF; Arg-1; AI256583
uniprot entry name :
ARGI1_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse ARG1. No significant cross-reactivity or interference between mouse ARG1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0. 312 mU/ml -20 mU/ml. Sensitivity: 0.078 mU/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ARG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ARG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ARG1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ARG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_031508.1
ncbi gb acc num :
NM_007482.3
ncbi mol weight :
34,808 Da
ncbi pathways :
Amoebiasis Pathway (167330); Amoebiasis Pathway (167191); Arginine And Proline Metabolism Pathway (83157); Arginine And Proline Metabolism Pathway (323); Metabolism Pathway (574739); Metabolism Of Amino Acids And Derivatives Pathway (574861); Polyamine Biosynthesis, Arginine = Ornithine = Putrescine Pathway (421800); Polyamine Biosynthesis, Arginine = Ornithine = Putrescine Pathway (468321); Urea Cycle Pathway (421751); Urea Cycle Pathway (574869)
uniprot summary :
ARG1: Homotrimer. By arginine or homoarginine. Belongs to the arginase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.5.3.1; Amino Acid Metabolism - arginine and proline; Hydrolase. Cellular Component: extracellular space; mitochondrial outer membrane; neuron projection; cell soma; cytoplasm; nucleus. Molecular Function: hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines; hydrolase activity; metal ion binding; manganese ion binding; arginase activity. Biological Process: arginine metabolic process; arginine catabolic process to ornithine; positive regulation of endothelial cell proliferation; collagen biosynthetic process; urea cycle
size4 :
10x96-Strip-Wells