Human Aldo-keto reductase family 1 member B10, AKR1B10 ELISA Kit | MyBioSource MBS935569 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Aldo-keto reductase family 1 member B10, AKR1B10 ELISA Kit

catalog :

MBS935569

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS935569

products type :

ELISA Kit

products full name :

Human Aldo-keto reductase family 1 member B10, AKR1B10 ELISA Kit

products short name :

aldo-keto reductase family 1, member B10 (aldose reductase)

products name syn :

Human Aldo-keto reductase family 1 member B10 (AKR1B10) ELISA kit; AKR1B11; AKR1B12; ALDRLn; ARL-1; ARL1; HIS; HSI; MGC14103; aldo-keto reductase family 1; member B10; aldo-keto reductase family 1; member B11 (aldose reductase-like) ; aldose reductase-like 1; aldose re; aldo-keto reductase family 1; member B10 (aldose reductase)

other names :

aldo-keto reductase family 1 member B10; Aldo-keto reductase family 1 member B10; aldo-keto reductase family 1 member B10; ARP; hARP; SI reductase; aldose reductase-like 1; small intestine reductase; aldose reductase-like peptide; aldose reductase-related protein; aldo-keto reductase family 1, member B11 (aldose reductase-like); aldo-keto reductase family 1, member B10 (aldose reductase); ARL-1; Aldose reductase-like; Aldose reductase-related protein; ARP; hARP; Small intestine reductase; SI reductase

products gene name :

AKR1B10

other gene names :

AKR1B10; AKR1B10; HIS; HSI; ARL1; ARL-1; ALDRLn; AKR1B11; AKR1B12; AKR1B11; ARP; hARP; SI reductase

uniprot entry name :

AK1BA_HUMAN

reactivity :

Human

sequence length :

316

specificity :

This assay has high sensitivity and excellent specificity for detection of human AKR1B10. No significant cross-reactivity or interference between human AKR1B10 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: The minimum detectable dose of human AKR1B10 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AKR1B10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AKR1B10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AKR1B10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AKR1B10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

223468663

ncbi acc num :

NP_064695.3

ncbi gb acc num :

NM_020299.4

uniprot acc num :

O60218

ncbi mol weight :

36,020 Da

ncbi pathways :

Fructose And Mannose Metabolism Pathway (82930); Fructose And Mannose Metabolism Pathway (291); Galactose Metabolism Pathway (82931); Galactose Metabolism Pathway (292); Glycerolipid Metabolism Pathway (82986); Glycerolipid Metabolism Pathway (361); Pentose And Glucuronate Interconversions Pathway (82929); Pentose And Glucuronate Interconversions Pathway (290); Pyruvate Metabolism Pathway (82998); Pyruvate Metabolism Pathway (373)

ncbi summary :

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis. [provided by RefSeq, Jul 2008]

uniprot summary :

AKR1B10: Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs. Belongs to the aldo/keto reductase family. Protein type: Carbohydrate Metabolism - fructose and mannose; EC 1.1.1.21; EC 1.1.1.-; Oxidoreductase; Lipid Metabolism - linoleic acid; Carbohydrate Metabolism - butanoate. Chromosomal Location of Human Ortholog: 7q33. Cellular Component: lysosome; cytosol. Molecular Function: protein binding; aldo-keto reductase activity; indanol dehydrogenase activity; geranylgeranyl reductase activity; retinal dehydrogenase activity. Biological Process: steroid metabolic process; phototransduction, visible light; digestion; aldehyde metabolic process; farnesol catabolic process; retinoid metabolic process

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage