Rat alpha-enolase ELISA Kit | MyBioSource MBS935486 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat alpha-enolase ELISA Kit

catalog :

MBS935486

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS935486

products type :

ELISA Kit

products full name :

Rat alpha-enolase ELISA Kit

products short name :

alpha-enolase

products name syn :

Rat alpha-enolase ELISA Kit; ENO1L1; MBP-1; MPB1; NNE; PPH; 2-phospho-D-glycerate hydro-lyase; MYC promoter-binding protein 1; alpha enolase like 1; enolase 1; non-neural enolase; phosphopyruvate hydratase; tau-crystallin; alpha-enolase

other names :

alpha-enolase; Alpha-enolase; alpha-enolase; alpha enolase; non-neural enolase; enolase 1, alpha non-neuron; 2-phospho-D-glycerate hydro-lyase; enolase 1, (alpha); 2-phospho-D-glycerate hydro-lyase; Enolase 1; Non-neural enolase; NNE

products gene name :

ENO1

other gene names :

Eno1; Eno1; Nne; Eno-1; NNE

uniprot entry name :

ENOA_RAT

reactivity :

Rat

sequence length :

434

specificity :

This assay has high sensitivity and excellent specificity for detection of rat alpha-enolase. No significant cross-reactivity or interference between rat alpha-enolase and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml - 40 ng/ml. Sensitivity: The minimum detectable dose of rat alpha-enolase is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for alpha-enolase has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any alpha-enolase present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for alpha-enolase is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of alpha-enolase bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

158186651

ncbi acc num :

NP_001103378.1

ncbi gb acc num :

NM_001109908.1

uniprot acc num :

P04764

ncbi mol weight :

47,128 Da

ncbi pathways :

Gluconeogenesis Pathway (573943); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (434661); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (468196); Glucose Metabolism Pathway (573941); Glycolysis Pathway (573942); Glycolysis (Embden-Meyerhof Pathway), Glucose = Pyruvate (434659); Glycolysis (Embden-Meyerhof Pathway), Glucose = Pyruvate (468194); Glycolysis / Gluconeogenesis Pathway (83319); Glycolysis / Gluconeogenesis Pathway (287); Glycolysis And Gluconeogenesis Pathway (198445)

uniprot summary :

ENO1: an abundant cytoplasmic enzyme with 2-phospho-D-glycerate hydro-lyase activity. A target of excess protein nitration in Alzheimer disease (AD) brain. Tau-crystallin, a structural lens protein, is produced from an alternative translation start in the enolase gene. Protein type: Transcription, coactivator/corepressor; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase; EC 4.2.1.11. Cellular Component: extrinsic to plasma membrane; extracellular space; neuron projection; phosphopyruvate hydratase complex; membrane; cytoplasm; nucleus. Molecular Function: protein homodimerization activity; RNA binding; protein heterodimerization activity; magnesium ion binding; phosphopyruvate hydratase activity. Biological Process: glycolysis; in utero embryonic development; response to virus; positive regulation of binding; negative regulation of cell growth; negative regulation of transcription, DNA-dependent

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage