ELISA/assay

Fish (Salmon) leptin, LEP ELISA Kit

MBS935480

48-Strip-Wells

565 USD

ELISA Kit

Fish (Salmon) leptin, LEP ELISA Kit

leptin (LEP)

Fish (Salmon) leptin (LEP) ELISA kit; leptin (LEP)

leptin; Leptin; leptin; obese protein; obesity factor; obese, mouse, homolog of; leptin (murine obesity homolog); leptin (obesity homolog, mouse); leptin; Obese protein; Obesity factor

LEP

LEP; LEP; OB; OBS; LEPD; OB; OBS

LEP_HUMAN

Salmon

167

This assay has high sensitivity and excellent specificity for detection of fish LEP. No significant cross-reactivity or interference between fish LEP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma. Assay Type: Competitive. Detection Range: 62.5 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of fish LEP is typically less than 31.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest fish LEP concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LEP. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated LEP. The competitive inhibition reaction is launched between with HRP-conjugated LEP and LEP in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of LEP in the sample. The color development is stopped and the intensity of the color is measured.

4557715

NP_000221.1

NM_000230.2

P41159

18,641 Da

Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Developmental Biology Pathway (477129); Diabetes Pathways (105902); Disease Pathway (530764); HIF-1-alpha Transcription Factor Network Pathway (138045); Incretin Synthesis, Secretion, And Inactivation Pathway (187170)

This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]

leptin: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in LEP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the leptin family. Protein type: Cell development/differentiation; Hormone; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7q31.3. Cellular Component: extracellular space; cytoplasm; extracellular region. Molecular Function: growth factor activity; peptide hormone receptor binding; hormone activity. Biological Process: circadian rhythm; response to dietary excess; positive regulation of myeloid cell differentiation; regulation of fat cell differentiation; regulation of steroid biosynthetic process; female pregnancy; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; positive regulation of luteinizing hormone secretion; negative regulation of appetite; response to insulin stimulus; positive regulation of tyrosine phosphorylation of Stat3 protein; response to vitamin E; positive regulation of MAPKKK cascade; regulation of cholesterol absorption; regulation of blood pressure; positive regulation of cell proliferation; positive regulation of ion transport; central nervous system neuron development; placenta development; positive regulation of cytokine production; cholesterol metabolic process; positive regulation of developmental growth; bile acid metabolic process; eating behavior; glucose metabolic process; adult feeding behavior; ovulation from ovarian follicle; leptin-mediated signaling pathway; negative regulation of vasoconstriction; tyrosine phosphorylation of STAT protein; fatty acid beta-oxidation; insulin secretion; glycerol biosynthetic process; response to hypoxia; energy reserve metabolic process; hormone metabolic process; regulation of gluconeogenesis; positive regulation of insulin receptor signaling pathway; positive regulation of follicle-stimulating hormone secretion; leukocyte tethering or rolling; regulation of insulin secretion; negative regulation of apoptosis. Disease: Leptin Deficiency

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150