ELISA/assay

Mouse DNA Damage-Inducible Transcript 4 (DDIT4) ELISA Kit

MBS9354284

48-Strip-Wells

470 USD

ELISA Kit

Mouse DNA Damage-Inducible Transcript 4 (DDIT4) ELISA Kit

[DNA Damage-Inducible Transcript 4 (DDIT4)]

[DNA damage-inducible transcript 4 protein; DNA damage-inducible transcript 4 protein; DNA damage-inducible transcript 4 protein; DNA damage inducible transcript 4; HIF-1 responsive protein RTP801; Protein regulated in development and DNA damage response 1; REDD-1]

[DDIT4]

[DDIT4; DDIT4; Dig2; REDD1; REDD-1; REDD1; RTP801; REDD-1]

DDIT4_HUMAN

Mouse

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Samples: undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. Assay Type: Quantitative Sandwich. Detection Range: 0.625 ng/ml - 20 ng/mL. Sensitivity: 0.1 ng/mL

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of DDIT4 (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

9506687

NP_061931.1

NM_019058.2

25,371 Da

Direct P53 Effectors Pathway (137939); Gene Expression Pathway (1269649); Generic Transcription Pathway (1269650); MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979); TOR Signaling Pathway (198762); TP53 Regulates Metabolic Genes Pathway (1269655); Transcriptional Regulation By TP53 Pathway (1269654)

DDIT4: Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death. Up-regulated in fibroblasts upon ionizing radiation, via a TP53-dependent pathway. Up-regulated by TP63 in primary keratinocytes, and down-regulated during keratinocyte differentiation. Up-regulated upon DNA alkylation. Up-regulated by amyloid beta-peptide and retinoic acid. Up-regulated by hypoxia, via a PI3K and HIF1A-dependent but TP53/TP63-independent mechanism. Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients. Belongs to the DDIT4 family. Chromosomal Location of Human Ortholog: 10q22.1. Cellular Component: cytoplasm; cytosol; intracellular; mitochondrion. Biological Process: brain development; cell proliferation; defense response to virus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; gene expression; negative regulation of glycolysis; negative regulation of peptidyl-serine phosphorylation; negative regulation of TOR signaling pathway; nerve growth factor receptor signaling pathway; neuron differentiation; neuron migration; protein complex disassembly; response to hypoxia; transcription initiation from RNA polymerase II promoter

48-Strip-Wells

470 USD

96-Strip-Wells

680

5x96-Strip-Wells

3100

10x96-Strip-Wells

6095