catalog number :
MBS935379
products type :
ELISA Kit
products full name :
Bovine Vascular endothelial growth factor A, VEGFA ELISA Kit
products short name :
vascular endothelial growth factor A
products name syn :
Bovine Vascular endothelial growth factor A (VEGFA) ELISA kit; RP1-261G23.1; MGC70609; MVCD1; VEGF; VEGF-A; VPF; vascular endothelial growth factor isoform VEGF165; vascular permeability factor; vascular endothelial growth factor A
other names :
vascular endothelial growth factor A; Vascular endothelial growth factor A; vascular endothelial growth factor A; vascular permeability factor; vascular endothelial growth factor 164; Vascular permeability factor; VPF
products gene name :
VEGFA
other gene names :
VEGFA; VEGFA; VPF; VEGF; VEGF-A; eVEGF120; eVEGF164; VEGF; VEGF-A; VPF
uniprot entry name :
VEGFA_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of bovine VEGFA. No significant cross-reactivity or interference between bovine VEGFA and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 47 pg/ml -3000 pg/ml. Sensitivity: 11.75 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VEGFA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGFA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGFA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGFA bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_776641.1
ncbi gb acc num :
NM_174216.1
ncbi mol weight :
22,310 Da
ncbi pathways :
Bladder Cancer Pathway (84350); Bladder Cancer Pathway (527); Focal Adhesion Pathway (84306); Focal Adhesion Pathway (478); Id Signaling Pathway (198561); Pancreatic Cancer Pathway (84343); Pancreatic Cancer Pathway (520); Pathways In Cancer (84340); Renal Cell Carcinoma Pathway (84342); Renal Cell Carcinoma Pathway (519)
uniprot summary :
Function: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin
size4 :
10x96-Strip-Wells