ELISA/assay

Rat Caspase-1, CASP1 ELISA Kit

MBS935341

48-Strip-Wells

510 USD

ELISA Kit

Rat Caspase-1, CASP1 ELISA Kit

caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)

Rat Caspase-1 (CASP1) ELISA kit; ICE; IL1BC; P45; CASP1 nirs variant 1; IL1B-convertase; caspase 1; caspase 1; apoptosis-related cysteine protease (interleukin 1; beta; convertase) ; caspase-1; interleukin 1-B converting enzyme; interleuk; caspase 1; apoptosis-related cysteine peptidase (interleukin 1; beta; convertase)

Caspase-1; Caspase-1; caspase-1; p45; CASP-1; IL-1BC; IL-1 beta-converting enzyme; interleukin-1 beta convertase; interleukin 1, beta, convertase; Interleukin 1beta converting enzyme; interleukin-1 beta-converting enzyme; caspase 1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45Cleaved into the following 2 chains:Caspase-1 subunit p20; Caspase-1 subunit p10

CASP1

Casp1; Casp1; Ice; Il1bc; Il1bc; Il1bce; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme

CASP1_RAT

Rat

402

This assay has high sensitivity and excellent specificity for detection of Rat CASP1. No significant cross-reactivity or interference between Rat CASP1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, Cell lysates, cell culture supernates. Assay Type: Sandwich. Detection Range: 6.25 pg/ml-400 pg/ml. Sensitivity: 1.56 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Apoptosis/Autophagy. Protein Biological Process 3: Apoptosis

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CASP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CASP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CASP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CASP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

1170463

P43527.1

P43527

45,576 Da

Amyotrophic Lateral Sclerosis (ALS) Pathway (83491); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Androgen Receptor Signaling Pathway (198529); Apoptosis Pathway (219785); Cytosolic DNA-sensing Pathway (125115); Cytosolic DNA-sensing Pathway (124833); Influenza A Pathway (217170); Influenza A Pathway (217150); Legionellosis Pathway (469194); Legionellosis Pathway (469186)

apoptotic protease that may play a role in inflammation and apoptosis in male sex organs [RGD, Feb 2006]

CASP1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis (By similarity). Protein type: Apoptosis; EC 3.4.22.36; Protease

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800