ELISA/assay

Mouse Glycogen [starch] synthase, liver, GYS2 ELISA Kit

MBS935152

48-Strip-Wells

510 USD

ELISA Kit

Mouse Glycogen [starch] synthase, liver, GYS2 ELISA Kit

glycogen synthase 2 (liver)

Mouse Glycogen [starch] synthase; liver (GYS2) ELISA kit; glycogen synthase 2; glycogen synthase 2 (liver)

glycogen; Glycogen [starch] synthase, liver; glycogen [starch] synthase, liver; glycogen [starch] synthase, liver; glycogen synthase, liver; glycogen synthase 2

GYS2

Gys2; Gys2; LGS; BC021322

GYS2_MOUSE

Mouse

704

This assay has high sensitivity and excellent specificity for detection of mouse GYS2. No significant cross-reactivity or interference between mouse GYS2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 12. 5 pg/ml -800 pg/ml. Sensitivity: The minimum detectable dose of mouse GYS2 is typically less than 3.12 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GYS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GYS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GYS2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GYS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

188035875

NP_663547.2

NM_145572.2

Q8VCB3

80,871 Da

Glucose Metabolism Pathway (574745); Glycogen Metabolism Pathway (198321); Glycogen Synthesis Pathway (574748); Insulin Signaling Pathway (198368); Insulin Signaling Pathway (83287); Insulin Signaling Pathway (501); Metabolism Pathway (574739); Metabolism Of Carbohydrates Pathway (574740); PI3K/Akt/mTOR Signaling Pathway (522982); Starch And Sucrose Metabolism Pathway (83173)

GYS2: Transfers the glycosyl residue from UDP-Glc to the non- reducing end of alpha-1,4-glucan. Defects in GYS2 are the cause of glycogen storage disease type 0 (GSD0); A metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood, high blood ketones and low alanine and lactate concentrations. Although feeding relieves symptoms, it often results in postprandial hyperglycemia and hyperlactatemia. Belongs to the glycosyltransferase 3 family. Protein type: EC 2.4.1.11; Carbohydrate Metabolism - starch and sucrose; Transferase; Cytoskeletal. Cellular Component: cortical actin cytoskeleton; cytoskeleton; ectoplasm; cytoplasm; cell cortex; cytosol. Molecular Function: transferase activity; transferase activity, transferring glycosyl groups; glycogen (starch) synthase activity; catalytic activity; glucose binding. Biological Process: glycogen metabolic process; glycogen biosynthetic process; metabolic process; response to glucose stimulus

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800