ELISA/assay

Human Age-related maculopathy susceptibility protein 2, ARMS2 ELISA Kit

MBS935086

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Human Age-related maculopathy susceptibility protein 2, ARMS2 ELISA Kit

[age-related maculopathy susceptibility 2]

[Human Age-related maculopathy susceptibility protein 2 (ARMS2) ELISA kit; ARMD8; ; age-related maculopathy susceptibility 2]

[age-related maculopathy susceptibility protein 2; Age-related maculopathy susceptibility protein 2; age-related maculopathy susceptibility protein 2; age-related maculopathy susceptibility 2]

[ARMS2]

[ARMS2; ARMS2; ARMD8]

ARMS2_HUMAN

Human

107

This assay has high sensitivity and excellent specificity for detection of human ARMS2. No significant cross-reactivity or interference between human ARMS2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of human ARMS2 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ARMS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ARMS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ARMS2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ARMS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

153792797

NP_001093137.1

NM_001099667.1

P0C7Q2

11,437 Da

This gene encodes a protein that is thought to play a role in diseases in the elderly. Mutations in this gene have been associated with age-related macular degeneration. [provided by RefSeq, Oct 2008]

ARMS2: Defects in ARMS2 influence susceptibility to age-related macular degeneration type 8 (ARMD8). ARMD is the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid (known as drusen) that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch s membrane. ARMD is likely to be a mechanistically heterogeneous group of disorders, and the specific disease mechanisms that underlie the vast majority of cases are currently unknown. However, a number of studies have suggested that both genetic and environmental factors are likely to play a role. Chromosomal Location of Human Ortholog: 10q26.13. Cellular Component: photoreceptor inner segment; mitochondrion. Biological Process: retinal homeostasis. Disease: Macular Degeneration, Age-related, 8

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

495

96-Strip-Wells

710

5x96-Strip-Wells

2705

10x96-Strip-Wells

5145