catalog number :
MBS935024
products type :
ELISA Kit
products full name :
Mouse Extracellular superoxide dismutase [Cu-Zn], SOD3 ELISA Kit
products short name :
[superoxide dismutase 3, extracellular]
products name syn :
[Mouse Extracellular superoxide dismutase [Cu-Zn] (SOD3) ELISA kit; EC-SOD; MGC20077; ; superoxide dismutase 3; extracellular]
other names :
[extracellular superoxide dismutase; Extracellular superoxide dismutase [Cu-Zn]; extracellular superoxide dismutase [Cu-Zn]; extracellular superoxide dismutase [Cu-Zn]; superoxide dismutase 3, extracellular]
products gene name :
[SOD3]
other gene names :
[Sod3; Sod3; EC-SOD; AI314465; EC-SOD]
uniprot entry name :
SODE_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of human SOCS-3. No significant cross-reactivity or interference between human SOCS-3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates. Assay Type: Quantitative Sandwich. Detection Range: 46.88 pg/ml-3000 pg/ml. Sensitivity: 11.75 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SOCS-3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SOCS-3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SOCS-3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SOCS-3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_035565.1
ncbi gb acc num :
NM_011435.3
ncbi mol weight :
27,392 Da
ncbi pathways :
One Carbon Metabolism And Related Pathways (198380); Oxidative Stress Pathway (198384); Superoxide Radicals Degradation Pathway (143050)
uniprot summary :
SOD3: Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. Belongs to the Cu-Zn superoxide dismutase family. Protein type: Secreted; Secreted, signal peptide; EC 1.15.1.1; Oxidoreductase. Cellular Component: extracellular matrix; extracellular space; cytoplasm; extracellular region; trans-Golgi network; nucleus. Molecular Function: antioxidant activity; protein binding; copper ion binding; zinc ion binding; metal ion binding; superoxide dismutase activity; oxidoreductase activity. Biological Process: response to copper ion; removal of superoxide radicals; response to hypoxia; superoxide metabolic process
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells