catalog number :
MBS935007
products type :
ELISA Kit
products full name :
Hamster Putative phospholipase B-like 2, PLBD2 ELISA Kit
products short name :
phospholipase B domain containing 2
products name syn :
Hamster Putative phospholipase B-like 2 (PLBD2) ELISA kit; P76; LAMA-like protein 2; PLB homolog 2; lamina ancestor homolog 2; mannose-6-phosphate protein associated protein p76; phospholipase B-like 2 32 kDa form; phospholipase B-like 2 45 kDa form; phospholipase B domain containing 2
other names :
putative phospholipase B-like 2 isoform 1; Putative phospholipase B-like 2; putative phospholipase B-like 2; PLB homolog 2; 76 kDa protein; LAMA-like protein 2; lamina ancestor homolog 2; phospholipase B-like 2 32 kDa form; phospholipase B-like 2 45 kDa form; phospholipase B domain-containing protein 2; mannose-6-phosphate protein associated protein p76; phospholipase B domain containing 2; 76 kDa protein; p76; LAMA-like protein 2; Lamina ancestor homolog 2; Phospholipase B domain-containing protein 2Cleaved into the following 2 chains:Putative phospholipase B-like 2 32 kDa form; Putative phospholipase B-like 2 45 kDa form
products gene name :
PLBD2
other gene names :
PLBD2; PLBD2; P76; p76
uniprot entry name :
PLBL2_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of hamster PLBD2. No significant cross-reactivity or interference between hamster PLBD2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates and cell lysates. Assay Type: Sandwich. Detection Range: 12.5 pg/ml -800 pg/ml. Sensitivity: 3.12 pg /ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLBD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLBD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLBD2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLBD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_775813.2
ncbi gb acc num :
NM_173542.3
ncbi mol weight :
61,885 Da
uniprot summary :
PLBL2: Putative phospholipase. Belongs to the phospholipase B-like family. Protein type: Phospholipase; EC 3.1.1.-. Chromosomal Location of Human Ortholog: 12q24.13. Cellular Component: lysosomal lumen. Molecular Function: hydrolase activity. Biological Process: lipid catabolic process
size4 :
10x96-Strip-Wells