Mouse Semaphorin-3A, SEMA3A ELISA Kit | MyBioSource MBS934985 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Semaphorin-3A, SEMA3A ELISA Kit

catalog :

MBS934985

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS934985

products type :

ELISA Kit

products full name :

Mouse Semaphorin-3A, SEMA3A ELISA Kit

products short name :

sema domain, immunoglobulin domain (Ig) , short basic domain, secreted, (semaphorin) 3A

products name syn :

Mouse Semaphorin-3A (SEMA3A) ELISA kit; Hsema-I; Hsema-III; MGC133243; SEMA1; SEMAD; SEMAIII; SEMAL; SemD; coll-1; OTTHUMP00000208884; collapsin 1; semaphorin 3A; semaphorin D; semaphorin III; semaphorin L; semaphorin-like; sema domain; immunoglobulin domain (Ig); short basic domain; secreted; (semaphorin) 3A

other names :

semaphorin-3A; Semaphorin-3A; semaphorin-3A; sema D; sema III; collapsin-1; semaphorin-D; semaphorin III; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A; Semaphorin III; Sema III; Semaphorin-D; Sema D

products gene name :

SEMA3A

other gene names :

Sema3a; Sema3a; SemD; SEMA1; Semad; coll-1; Hsema-I; Semad; SemD; Sema III; Sema D

uniprot entry name :

SEM3A_MOUSE

reactivity :

Mouse

sequence length :

772

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse SEMA3A. No significant cross-reactivity or interference between mouse SEMA3A and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of mouse SEMA3A is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SEMA3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEMA3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA3A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA3A bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

340523100

ncbi acc num :

NP_001230001.1

ncbi gb acc num :

NM_001243072.1

uniprot acc num :

O08665

ncbi mol weight :

88,813 Da

ncbi pathways :

Axon Guidance Pathway (83262); Axon Guidance Pathway (476); Axon Guidance Pathway (575211); CRMPs In Sema3A Signaling Pathway (575218); Developmental Biology Pathway (575210); SEMA3A-Plexin Repulsion Signaling By Inhibiting Integrin Adhesion Pathway (575216); Sema3A PAK Dependent Axon Repulsion Pathway (575217); Semaphorin Interactions Pathway (575212)

uniprot summary :

SEMA3A: Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. Belongs to the semaphorin family. Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis. Cellular Component: membrane; axon; dendrite; extracellular region. Molecular Function: chemorepellent activity; receptor activity; semaphorin receptor binding. Biological Process: trigeminal nerve structural organization; nervous system development; facial nerve structural organization; axon guidance; apoptosis; regulation of heart rate; olfactory bulb development; dendrite morphogenesis; multicellular organismal development; neuron migration; axonal fasciculation; negative chemotaxis; motor axon guidance; negative regulation of axon extension; negative regulation of axon extension involved in axon guidance; axon extension involved in axon guidance; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; branchiomotor neuron axon guidance; regulation of axon extension involved in axon guidance; nerve development; cell differentiation

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage