Mouse Group XIIA secretory phospholipase A2, PLA2G12A ELISA Kit | MyBioSource MBS934962 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Group XIIA secretory phospholipase A2, PLA2G12A ELISA Kit

catalog :

MBS934962

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS934962

products type :

ELISA Kit

products full name :

Mouse Group XIIA secretory phospholipase A2, PLA2G12A ELISA Kit

products short name :

phospholipase A2, group XIIA

products name syn :

Mouse Group XIIA secretory phospholipase A2 (PLA2G12A) ELISA kit; GXII; PLA2G12; ROSSY; group XII secreted phospholipase A2; group XIIA secreted phospholipase A2; phospholipase A2; group XIIA

other names :

group XIIA secretory phospholipase A2 isoform 1; Group XIIA secretory phospholipase A2; group XIIA secretory phospholipase A2; group XII-1 phospholipase A2; group XIIA secreted phospholipase A2; phosphatidylcholine 2-acylhydrolase 12A; phospholipase A2, group XIIA; Phosphatidylcholine 2-acylhydrolase 12A

products gene name :

PLA2G12A

other gene names :

Pla2g12a; Pla2g12a; GXII; Rossy; GXII-1; Pla2g12; mGXII-1; GXII-1-PLA2; 2310004B05Rik; Pla2g12; GXII sPLA2; sPLA2-XII

uniprot entry name :

PG12A_MOUSE

reactivity :

Mouse

sequence length :

192

specificity :

This assay has high sensitivity and excellent specificity for detection of Mouse PLA2G12A. No significant cross-reactivity or interference between Mouse PLA2G12A and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml-4000 pg/ml. Sensitivity: 15.6 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: Lipogenesis and lipometabolism. Protein Biological Process 3: Lipid degradation

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLA2G12A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLA2G12A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA2G12A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA2G12A bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

170295846

ncbi acc num :

NP_075685.2

ncbi gb acc num :

NM_023196.4

uniprot acc num :

Q9EPR2

ncbi mol weight :

21,319 Da

ncbi pathways :

Arachidonic Acid Metabolism Pathway (83190); Arachidonic Acid Metabolism Pathway (366); Ether Lipid Metabolism Pathway (83189); Ether Lipid Metabolism Pathway (365); Fat Digestion And Absorption Pathway (194386); Fat Digestion And Absorption Pathway (194324); Fc Epsilon RI Signaling Pathway (83279); Fc Epsilon RI Signaling Pathway (493); Glutamatergic Synapse Pathway (213819); Glutamatergic Synapse Pathway (213811)

uniprot summary :

PLA2G12A: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl- phosphatidylcholine or -phosphatidylethanolamine. Belongs to the phospholipase A2 family. Protein type: EC 3.1.1.4; Lipid Metabolism - glycerophospholipid; Secreted, signal peptide; Lipid Metabolism - ether lipid; Lipid Metabolism - linoleic acid; Secreted; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - arachidonic acid; Phospholipase; Endoplasmic reticulum. Cellular Component: Golgi apparatus; endoplasmic reticulum; cytoplasm; extracellular region; intracellular. Molecular Function: phospholipase A2 activity; hydrolase activity; metal ion binding; calcium ion binding. Biological Process: phospholipid metabolic process; lipid metabolic process; lipid catabolic process

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage