catalog number :
MBS934900
products type :
ELISA Kit
products full name :
Mouse Inter-alpha-trypsin inhibitor heavy chain H3, ITIH3 ELISA Kit
products short name :
inter-alpha (globulin) inhibitor H3
products name syn :
Mouse Inter-alpha-trypsin inhibitor heavy chain H3 (ITIH3) ELISA kit; H3P; inter-alpha (globulin) inhibitor; H3 polypeptide; inter-alpha-trypsin inhibitor heavy chain H3; pre-alpha (globulin) inhibitor; H3 polypeptide; inter-alpha (globulin) inhibitor H3
other names :
inter-alpha-trypsin inhibitor heavy chain H3; Inter-alpha-trypsin inhibitor heavy chain H3; inter-alpha-trypsin inhibitor heavy chain H3; ITI-HC3; ITI heavy chain H3; inter-alpha-inhibitor H3 chain; inter-alpha-inhibitor heavy chain 3; inter-alpha trypsin inhibitor, heavy chain 3
products gene name :
ITIH3
other gene names :
Itih3; Itih3; Intin3; Itih-3; AW108094; ITI heavy chain H3; ITI-HC3; Inter-alpha-inhibitor heavy chain 3
uniprot entry name :
ITIH3_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse ITIH3. No significant cross-reactivity or interference between mouse ITIH3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 ng/ml -1000 ng/ml. Sensitivity: The minimum detectable dose of mouse ITIH3 is typically less than 3.9 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ITIH3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ITIH3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ITIH3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ITIH3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_032433.2
ncbi gb acc num :
NM_008407.2
ncbi mol weight :
99,358 Da
uniprot summary :
ITIH3: May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes. Belongs to the ITIH family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Inhibitor; Secreted; Secreted, signal peptide. Cellular Component: extracellular region. Molecular Function: serine-type endopeptidase inhibitor activity; protease inhibitor activity. Biological Process: negative regulation of peptidase activity; hyaluronan metabolic process
size4 :
10x96-Strip-Wells