product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Interleukin-27 subunit beta, EBI3 ELISA Kit
catalog :
MBS934823
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS934823
products type :
ELISA Kit
products full name :
Mouse Interleukin-27 subunit beta, EBI3 ELISA Kit
products short name :
Epstein-Barr virus induced 3
products name syn :
Mouse Interleukin-27 subunit beta (EBI3) ELISA kit; IL27B; Epstein-Barr virus induced gene 3; cytokine receptor; interleukin-27 subunit beta; Epstein-Barr virus induced 3
other names :
interleukin-27 subunit beta; Interleukin-27 subunit beta; interleukin-27 subunit beta; IL-27B; IL-27 subunit beta; epstein-Barr virus-induced gene 3 protein homolog; Epstein-Barr virus induced gene 3; Epstein-Barr virus-induced gene 3 protein homolog
products gene name :
EBI3
other gene names :
Ebi3; Ebi3; EBI-3; IL-27; Il27b; IL-27 subunit beta; IL-27B
uniprot entry name :
IL27B_MOUSE
reactivity :
Mouse
sequence length :
228
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse EBI3. No significant cross-reactivity or interference between mouse EBI3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.87 pg/ml-120 pg/ml. Sensitivity: The minimum detectable dose of mouse EBI3 is typically less than 0.46 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EBI3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EBI3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EBI3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EBI3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
7657052
ncbi acc num :
NP_056581.1
ncbi gb acc num :
NM_015766.2
uniprot acc num :
O35228
ncbi mol weight :
25,353 Da
uniprot summary :
IL27-beta: Cytokine with pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon- gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines. Belongs to the type I cytokine receptor family. Type 3 subfamily. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular space; membrane; extracellular region. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; interleukin-27 receptor binding; cytokine activity. Biological Process: cytokine and chemokine mediated signaling pathway
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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