catalog number :
MBS934816
products type :
ELISA Kit
products full name :
Mouse Lymphocyte activation gene 3 protein, LAG3 ELISA Kit
products short name :
lymphocyte-activation gene 3
products name syn :
Mouse Lymphocyte activation gene 3 protein (LAG3) ELISA kit; CD223; lymphocyte-activation protein 3; lymphocyte-activation gene 3
other names :
lymphocyte activation gene 3 protein; Lymphocyte activation gene 3 protein; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; CD_antigen: CD223
products gene name :
LAG3
other gene names :
Lag3; Lag3; Ly66; CD223; LAG-3; LAG-3
uniprot entry name :
LAG3_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse LAG3. No significant cross-reactivity or interference between mouse LAG3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.25 ng/ml -80 ng/ml. Sensitivity: The minimum detectable dose of mouse LAG3 is typically less than 0.31 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LAG3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LAG3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAG3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAG3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_032505.1
ncbi gb acc num :
NM_008479.2
ncbi mol weight :
56,978 Da
uniprot summary :
LAG3: Involved in lymphocyte activation. Binds to HLA class-II antigens. Protein type: Membrane protein, integral. Cellular Component: membrane; integral to membrane; external side of plasma membrane. Molecular Function: transmembrane receptor activity; MHC class II protein binding. Biological Process: cell surface receptor linked signal transduction; negative regulation of interleukin-2 biosynthetic process; positive regulation of natural killer cell mediated cytotoxicity; negative regulation of T cell activation
size4 :
10x96-Strip-Wells