ELISA/assay

Mouse Disintegrin and metalloproteinase domain-containing protein 17, ADAM17 ELISA Kit

MBS934765

48-Strip-Wells

510 USD

ELISA Kit

Mouse Disintegrin and metalloproteinase domain-containing protein 17, ADAM17 ELISA Kit

ADAM metallopeptidase domain 17

Mouse Disintegrin and metalloproteinase domain-containing protein 17 (ADAM17) ELISA kit; ADAM18; CD156B; CSVP; MGC71942; TACE; ADAM metallopeptidase domain 18; TNF-alpha converting enzyme; a disintegrin and metalloprotease domain 17; snake venom-like protease; tumor necrosis factor; alpha; ; ADAM metallopeptidase domain 17

disintegrin and metalloproteinase domain-containing protein 17 isoform 2; Disintegrin and metalloproteinase domain-containing protein 17; disintegrin and metalloproteinase domain-containing protein 17; TNF-alpha convertase; TNF-alpha converting enzyme; a disintegrin and metalloproteinase domain 17; a disintegrin and metallopeptidase domain 17; TNF-alpha convertase; TNF-alpha-converting enzyme; CD_antigen: CD156b

ADAM17

Adam17; Adam17; Tace; CD156b; Tace; ADAM 17

ADA17_MOUSE

Mouse

827

This assay has high sensitivity and excellent specificity for detection of mouse ADAM17. No significant cross-reactivity or interference between mouse ADAM17 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: 3.9 pg /ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAM17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAM17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM17 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM17 bound in the initial step. The color development is stopped and the intensity of the color is measured.

471270257

NP_001264195.1

NM_001277266.1

Q9Z0F8

93,056 Da

Alzheimer's Disease Pathway (83294); Alzheimer's Disease Pathway (509); Delta-Notch Signaling Pathway (198316); Disease Pathway (574923); Notch Signaling Pathway (198301); Notch Signaling Pathway (83259); Notch Signaling Pathway (473); Regulated Proteolysis Of P75NTR Pathway (575377); Signal Transduction Pathway (575318); Signaling By EGFR Pathway (574925)

TACE: a type I membrane protein with disintegrin and metalloprotease activity. An ubiquitously expressed ectoenzyme of peptidase family M12B. Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Possesses a narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala- -Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha) to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, and the amyloid precursor protein. Also involved in the activation of Notch pathway. Binds 1 zinc ion per subunit. Two splice variant isoforms have been described. Protein type: Motility/polarity/chemotaxis; Protease; Membrane protein, integral; EC 3.4.24.86. Cellular Component: cell surface; focal adhesion; membrane; integral to plasma membrane; cytoplasm; apical plasma membrane; extracellular region; plasma membrane; integral to membrane; intercellular junction; actin cytoskeleton; lipid raft. Molecular Function: integrin binding; peptidase activity; protein binding; interleukin-6 receptor binding; zinc ion binding; metallopeptidase activity; hydrolase activity; metalloendopeptidase activity; metal ion binding; Notch binding; SH3 domain binding; PDZ domain binding. Biological Process: cell motility involved in cell locomotion; T cell differentiation in the thymus; response to lipopolysaccharide; proteolysis; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; negative regulation of interleukin-8 production; germinal center formation; cell adhesion; epidermal growth factor receptor signaling pathway; response to drug; spleen development; Notch signaling pathway; response to high density lipoprotein stimulus; membrane protein ectodomain proteolysis; positive regulation of transforming growth factor beta receptor signaling pathway; Notch receptor processing; positive regulation of cell motility; positive regulation of cell growth; positive regulation of chemokine production; regulation of mast cell apoptosis; PMA-inducible membrane protein ectodomain proteolysis; B cell differentiation; response to hypoxia; cell adhesion mediated by integrin; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of apoptosis; positive regulation of cell migration

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800