ELISA/assay

Bovine C-C motif chemokine 20, CCL20 ELISA Kit

MBS934740

48-Strip-Wells

565 USD

ELISA Kit

Bovine C-C motif chemokine 20, CCL20 ELISA Kit

chemokine (C-C motif) ligand 20

Bovine C-C motif chemokine 20 (CCL20) ELISA kit; CKb4; LARC; MIP-3a; MIP3A; SCYA20; ST38; exodus-1; macrophage inflammatory protein 3 alpha; small inducible cytokine subfamily A (Cys-Cys); member 20; chemokine (C-C motif) ligand 20

C-C motif chemokine 20; C-C motif chemokine 20; C-C motif chemokine 20; MIP-3-alpha; small-inducible cytokine A20; macrophage inflammatory protein 3 alpha; chemokine (C-C motif) ligand 20; Macrophage inflammatory protein 3 alpha; MIP-3-alpha; Small-inducible cytokine A20

CCL20

CCL20; CCL20; MIP3A; SCYA20; MIP-3-alpha

CCL20_BOVIN

Bovine

96

This assay has high sensitivity and excellent specificity for detection of bovine CCL20. No significant cross-reactivity or interference between bovine CCL20 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of bovine CCL20 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CCL20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL20 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL20 bound in the initial step. The color development is stopped and the intensity of the color is measured.

27806273

NP_776688.1

NM_174263.2

Q8SQB1

10,964 Da

Chemokine Receptors Bind Chemokines Pathway (581438); Chemokine Signaling Pathway (98761); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (581436); Cytokine-cytokine Receptor Interaction Pathway (84290); Cytokine-cytokine Receptor Interaction Pathway (460); G Alpha (i) Signalling Events Pathway (581463); GPCR Downstream Signaling Pathway (581461); GPCR Ligand Binding Pathway (581435); Peptide Ligand-binding Receptors Pathway (581437)

Function: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150