catalog number :
MBS934713
products type :
ELISA Kit
products full name :
Mouse Chymase, CMA1 ELISA Kit
products short name :
chymase 1, mast cell
products name syn :
Mouse Chymase (CMA1) ELISA kit; CYH; MCT1; MGC119890; MGC119891; chymase; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; chymase; heart; chymase; mast cell; mast cell protease I; chymase 1; mast cell
other names :
chymase; Chymase; chymase; alpha-chymase; mast cell chymase 1; mast cell protease 5; mast cell protease I; chymase 1, mast cell; Alpha-chymase; Mast cell chymase 1; Mast cell protease 5; mMCP-5; Mast cell protease I
products gene name :
CMA1
other gene names :
Cma1; Cma1; Mcp5; Mcp-5; Mcpt5; MMCP-5; Mcpt5; mMCP-5
uniprot entry name :
CMA1_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse CMA1. No significant cross-reactivity or interference between Mouse CMA1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156ng/ml-10 ng/ml. Sensitivity: 0.039ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for chymase has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any chymase present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for chymase is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of chymase bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_034910.1
ncbi gb acc num :
NM_010780.2
ncbi mol weight :
27,586 Da
ncbi pathways :
Renin-angiotensin System Pathway (83272); Renin-angiotensin System Pathway (486)
uniprot summary :
CMA1: Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. Belongs to the peptidase S1 family. Granzyme subfamily. Protein type: Secreted, signal peptide; EC 3.4.21.39; Secreted; Protease. Cellular Component: extracellular matrix; extracellular space; extracellular region; intracellular. Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity; peptide binding. Biological Process: interleukin-1 beta biosynthetic process; positive regulation of angiogenesis; protein processing; proteolysis
size4 :
10x96-Strip-Wells