product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Pig Toll-like receptor 4, TLR4 ELISA Kit
catalog :
MBS934418
quantity :
48-Strip-Wells
price :
565 USD
more info or order :
product information
catalog number :
MBS934418
products type :
ELISA Kit
products full name :
Pig Toll-like receptor 4, TLR4 ELISA Kit
products short name :
toll-like receptor 4
products name syn :
Pig Toll-like receptor 4 (TLR4) ELISA kit; ARMD10; CD284; TOLL; hToll; homolog of Drosophila toll; toll-like receptor 4
other names :
Toll-like receptor 4; Toll-like receptor 4; toll-like receptor 4; CD_antigen: CD284
products gene name :
TLR4
other gene names :
TLR4; TLR4
uniprot entry name :
TLR4_PIG
reactivity :
Pig
sequence length :
841
specificity :
This assay has high sensitivity and excellent specificity for detection of pig TLR4. No significant cross-reactivity or interference between pig TLR4 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 ng/ml -200 ng/ml. Sensitivity: The minimum detectable dose of pig TLR4 is typically less than 0.78 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TLR4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TLR4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLR4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLR4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
62903518
ncbi acc num :
Q68Y56.1
uniprot acc num :
Q68Y56
ncbi mol weight :
96,309 Da
ncbi pathways :
Amoebiasis Pathway (167313); Amoebiasis Pathway (167191); Chagas Disease (American Trypanosomiasis) Pathway (147807); Chagas Disease (American Trypanosomiasis) Pathway (147795); Influenza A Pathway (217171); Influenza A Pathway (217150); Legionellosis Pathway (469196); Legionellosis Pathway (469186); Leishmaniasis Pathway (144174); Leishmaniasis Pathway (144028)
uniprot summary :
Function: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response
size1 :
48-Strip-Wells
price1 :
565 USD
size2 :
96-Strip-Wells
price2 :
810
size3 :
5x96-Strip-Wells
price3 :
2750
size4 :
10x96-Strip-Wells
price4 :
5150
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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