ELISA/assay

Human Tryptase delta, TPSD1 ELISA Kit

MBS934410

48-Strip-Wells

510 USD

ELISA Kit

Human Tryptase delta, TPSD1 ELISA Kit

tryptase delta 1

Human Tryptase delta (TPSD1) ELISA kit; MCP7-LIKE; MCP7L1; MGC95428; MMCP-7L; hmMCP-3-like tryptase III; hmMCP-7-like; mMCP-7-like delta II tryptase; mMCP-7-like-1; mMCP-7-like-2; mast cell protease 7-like; mast cell tryptase; tryptase delta 1

tryptase delta; Tryptase delta; tryptase delta; tryptase-3; mMCP-7-like-1; mMCP-7-like-2; delta-tryptase; mast cell tryptase; hmMCP-3-like tryptase III; mMCP-7-like delta II tryptase; tryptase delta 1; Delta-tryptase; HmMCP-3-like tryptase III; Mast cell mMCP-7-like; Tryptase-3

TPSD1

TPSD1; TPSD1; MCP7L1; MMCP-7L; MCP7-LIKE

TRYD_HUMAN

Human

242

This assay has high sensitivity and excellent specificity for detection of human TPSD1. No significant cross-reactivity or interference between human TPSD1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: 0.039 ng /ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TPSD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPSD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPSD1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPSD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

11321636

NP_036349.1

NM_012217.2

Q9BZJ3

26,584 Da

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. Although this gene may be an exception, most of the tryptase genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. This gene was once considered to be a pseudogene, although it is now believed to be a functional gene that encodes a protein. [provided by RefSeq, Jul 2008]

TPSD1: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Belongs to the peptidase S1 family. Tryptase subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.21.59; Secreted, signal peptide; Secreted; Protease. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: extracellular region. Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity. Biological Process: proteolysis

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800