catalog number :
MBS9343383
products type :
ELISA Kit
products full name :
Human von Willebrand factor A domain-containing protein 5B2, VWA5B2 ELISA Kit
products short name :
von Willebrand factor A domain containing 5B2
products name syn :
Human von Willebrand factor A domain-containing protein 5B2 (VWA5B2) ELISA kit; von Willebrand factor A domain containing 5B2
other names :
von Willebrand factor A domain-containing protein 5B2; von Willebrand factor A domain-containing protein 5B2; von Willebrand factor A domain-containing protein 5B2; von Willebrand factor A domain containing 5B2
products gene name :
VWA5B2
other gene names :
VWA5B2; VWA5B2
uniprot entry name :
VW5B2_HUMAN
specificity :
No significant cross-reactivity or interference between Human VWA5B2 and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 62.5 pg/ml - 2000 pg/ml. Sensitivity: 10 pg/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated VWA5B2 concentrations in Human serum, plasma and other body fluids. Using Purified Human VWA5B2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add VWA5B2 and VWA5B2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of VWA5B2 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated VWA5B2 concentrations in Human serum, plasma and other body fluids. Using Purified Human VWA5B2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add VWA5B2 and VWA5B2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of VWA5B2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_612354.1
ncbi gb acc num :
NM_138345.1
ncbi mol weight :
132,764 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
VWA5B2: 2 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: 3q27.1