ELISA/assay

Human Sperm-associated antigen 5, SPAG5 ELISA Kit

MBS9342448

48-Strip-Wells

435 USD

ELISA Kit

Human Sperm-associated antigen 5, SPAG5 ELISA Kit

sperm associated antigen 5

Human Sperm-associated antigen 5 (SPAG5) ELISA kit; DEEPEST; MAP126; hMAP126; astrin; mitotic spindle associated protein p126; mitotic spindle coiled-coil related protein; sperm associated antigen 5

sperm-associated antigen 5; Sperm-associated antigen 5; sperm-associated antigen 5; astrin; mitotic spindle associated protein p126; mitotic spindle-associated protein p126; mitotic spindle coiled-coil related protein; sperm associated antigen 5; Astrin; Deepest; Mitotic spindle-associated protein p126; MAP126

SPAG5

SPAG5; SPAG5; MAP126; DEEPEST; hMAP126; MAP126

SPAG5_HUMAN

Human

No significant cross-reactivity or interference between Human SPAG5 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SPAG5 concentrations in Human serum, plasma and other body fluids. Using Purified Human SPAG5 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SPAG5 and SPAG5 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SPAG5 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SPAG5 concentrations in Human serum, plasma and other body fluids. Using Purified Human SPAG5 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SPAG5 and SPAG5 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SPAG5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

73623035

NP_006452.3

NM_006461.3

Q96R06

134,422 Da

This gene encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functional and dynamic regulation of mitotic spindles. [provided by RefSeq, Jul 2008]

SPAG5: Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. The astrin (SPAG5)-kinastrin (SKAP) complex promotes stable microtubule-kinetochore attachments. Protein type: Cell cycle regulation. Chromosomal Location of Human Ortholog: 17q11.2. Cellular Component: kinetochore; nucleoplasm; spindle pole; cytoplasm; microtubule organizing center; midbody; nucleus. Molecular Function: protein binding. Biological Process: regulation of attachment of spindle microtubules to kinetochore; mitotic sister chromatid segregation; cell division; spindle organization and biogenesis; chromosome segregation

48-Strip-Wells

435 USD

96-Strip-Wells

600