catalog number :
MBS9341972
products type :
ELISA Kit
products full name :
Human Sulfiredoxin-1, SRXN1 ELISA Kit
products short name :
sulfiredoxin 1 homolog (S. cerevisiae)
products name syn :
Human Sulfiredoxin-1 (SRXN1) ELISA kit; C20orf139; Npn3; SRX1; YKL086W; dJ850E9.2; sulfiredoxin 1 homolog; sulfiredoxin 1 homolog (S. cerevisiae)
other names :
sulfiredoxin-1; Sulfiredoxin-1; sulfiredoxin-1; sulfiredoxin 1
products gene name :
SRXN1
other gene names :
SRXN1; SRXN1; SRX; Npn3; SRX1; C20orf139; C20orf139; SRX
uniprot entry name :
SRXN1_HUMAN
specificity :
No significant cross-reactivity or interference between Human SRXN1 and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SRXN1 concentrations in Human serum, plasma and other body fluids. Using Purified Human SRXN1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SRXN1 and SRXN1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SRXN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SRXN1 concentrations in Human serum, plasma and other body fluids. Using Purified Human SRXN1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SRXN1 and SRXN1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SRXN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_542763.1
ncbi gb acc num :
NM_080725.2
ncbi mol weight :
14,259 Da
uniprot summary :
SRXN1: Contributes to oxidative stress resistance by reducing cysteine-sulfinic acid formed under exposure to oxidants in the peroxiredoxins PRDX1, PRDX2, PRDX3 and PRDX4. Does not act on PRDX5 or PRDX6. May catalyze the reduction in a multi-step process by acting both as a specific phosphotransferase and a thioltransferase. Belongs to the sulfiredoxin family. Protein type: EC 1.8.98.2; Oxidoreductase. Chromosomal Location of Human Ortholog: 20p13. Cellular Component: cytosol. Molecular Function: oxidoreductase activity, acting on sulfur group of donors; sulfiredoxin activity; ATP binding. Biological Process: response to oxidative stress