Mouse Integrin alpha-M, Itgam/CD11b ELISA Kit | MyBioSource MBS934157 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Integrin alpha-M, Itgam/CD11b ELISA Kit

catalog :

MBS934157

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS934157

products type :

ELISA Kit

products full name :

Mouse Integrin alpha-M, Itgam/CD11b ELISA Kit

products short name :

Integrin alpha-M (Itgam/CD11b)

products name syn :

Mouse Integrin alpha-M (Itgam/CD11b) ELISA Kit; CD11B; CR3A; MAC-1; MAC1A; MGC117044; MO1A; SLEB6; antigen CD11b (p170) ; integrin alpha M; macrophage antigen alpha polypeptide; neutrophil adherence receptor alpha-M subunit; Integrin alpha-M (Itgam/CD11b)

other names :

Integrin alpha-M; Integrin alpha-M; integrin alpha-M; Mac-1 alpha; CD11B (p170); CR-3 alpha chain; macrophage antigen alpha; complement receptor type 3; leukocyte adhesion receptor MO1; CD11 antigen-like family member B; complement component receptor 3 alpha-a; cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; integrin alpha M; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; CD_antigen: CD11b

products gene name :

Itgam/CD11b

other gene names :

Itgam; Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik

uniprot entry name :

ITAM_MOUSE

reactivity :

Mouse

sequence length :

1,153

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse Itgam/CD11b. No significant cross-reactivity or interference between mouse Itgam/CD11b and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell culture supernates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: The minimum detectable dose of mouse Itgam/CD11b is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Itgam/CD11b has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Itgam/CD11b present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Itgam/CD11b is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Itgam/CD11b bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

124956

ncbi acc num :

P05555.2

uniprot acc num :

P05555

ncbi mol weight :

127,481 Da

ncbi pathways :

Amoebiasis Pathway (167330); Amoebiasis Pathway (167191); Cell Adhesion Molecules (CAMs) Pathway (83266); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (575507); Focal Adhesion Pathway (198353); Hematopoietic Cell Lineage Pathway (83275); Hematopoietic Cell Lineage Pathway (489); Hemostasis Pathway (575479); IL-5 Signaling Pathway (198281)

uniprot summary :

ITGAM: a single-pass type I membrane protein of the integrin alpha chain family. Implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. Also known as CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. A receptor for fibrinogen, factor X and ICAM1. Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2. Predominantly expressed in monocytes and granulocytes. Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion. Cellular Component: cell surface; membrane; integral to membrane; integrin complex; nucleus; external side of plasma membrane. Molecular Function: heparin binding; heparan sulfate proteoglycan binding; opsonin binding; protein heterodimerization activity; metal ion binding; glycoprotein binding. Biological Process: integrin-mediated signaling pathway; neutrophil chemotaxis; cell-cell adhesion; leukocyte adhesion; microglia development; cellular extravasation; activated T cell proliferation; cell adhesion; leukocyte migration during inflammatory response

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage