ELISA/assay

Mouse Prestin, SLC26A5 ELISA Kit

MBS9340464

48-Strip-Wells

470 USD

ELISA Kit

Mouse Prestin, SLC26A5 ELISA Kit

[solute carrier family 26, member 5 (prestin)]

[Mouse Prestin (SLC26A5) ELISA kit; DFNB61; MGC118886; MGC118887; MGC118888; MGC118889; PRES; deafness; neurosensory; autosomal recessive; 61; prestin; prestin (motor protein) ; solute carrier family 26; member 5 (prestin)]

[prestin isoform 2; Prestin; prestin; prestin (motor protein); outer hair cell motor protein; solute carrier family 26, member 5; Solute carrier family 26 member 5]

[SLC26A5]

[Slc26a5; Slc26a5; Pres; prestin; Pres]

S26A5_MOUSE

Mouse

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Assay Type: Quantitative Sandwich. Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of SLC26A5 (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

577019546

NP_001276716.1

NM_001289787.1

Q99NH7

81,337 Da

SLC26A5: Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage- to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane. The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site. Defects in SLC26A5 are the cause of deafness autosomal recessive type 61 (DFNB61). A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the SLC26A/SulP transporter (TC 2.A.53) family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter; Transporter, SLC family. Cellular Component: membrane; basolateral plasma membrane; cytoplasm; integral to membrane; plasma membrane; lateral plasma membrane. Molecular Function: protein binding; protein homodimerization activity; spectrin binding; sulfate transmembrane transporter activity; secondary active sulfate transmembrane transporter activity; transcription factor binding. Biological Process: regulation of cell shape; regulation of membrane potential; sensory perception of sound; positive regulation of cell size; fructose transport; sulfate transport; transmembrane transport; protein tetramerization

48-Strip-Wells

470 USD

96-Strip-Wells

680

5x96-Strip-Wells

3100

10x96-Strip-Wells

6095