Human Serine--pyruvate aminotransferase, AGXT ELISA Kit | MyBioSource MBS934004 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Serine--pyruvate aminotransferase, AGXT ELISA Kit

catalog :

MBS934004

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS934004

products type :

ELISA Kit

products full name :

Human Serine--pyruvate aminotransferase, AGXT ELISA Kit

products short name :

alanine-glyoxylate aminotransferase

products name syn :

Human Serine--pyruvate aminotransferase (AGXT) ELISA kit; AGT; AGT1; AGXT1; PH1; SPAT; SPT; TLH6; L-alanine: glyoxylate aminotransferase 1; hepatic peroxisomal alanine:glyoxylate aminotransferase; serine-pyruvate aminotransferase; serine:pyruvate aminotransfe; alanine-glyoxylate aminotransferase

other names :

serine--pyruvate aminotransferase; Serine--pyruvate aminotransferase; serine--pyruvate aminotransferase; serine-pyruvate aminotransferase; serine:pyruvate aminotransferase; alanine--glyoxylate aminotransferase; L-alanine: glyoxylate aminotransferase 1; hepatic peroxisomal alanine:glyoxylate aminotransferase; alanine-glyoxylate aminotransferase; Alanine--glyoxylate aminotransferase (EC:2.6.1.44); AGT

products gene name :

AGXT

other gene names :

AGXT; AGXT; AGT; PH1; SPT; AGT1; SPAT; TLH6; AGXT1; AGT1; SPAT; SPT; AGT

uniprot entry name :

SPYA_HUMAN

reactivity :

Human

sequence length :

392

specificity :

This assay has high sensitivity and excellent specificity for detection of human AGXT. No significant cross-reactivity or interference between human AGXT and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of human AGXT is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AGXT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AGXT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGXT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGXT bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4557289

ncbi acc num :

NP_000021.1

ncbi gb acc num :

NM_000030.2

uniprot acc num :

P21549

ncbi mol weight :

43,010 Da

ncbi pathways :

Alanine And Aspartate Metabolism Pathway (198783); Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Glycine, Serine And Threonine Metabolism Pathway (82949); Glycine, Serine And Threonine Metabolism Pathway (313); Glyoxylate And Dicarboxylate Metabolism Pathway (83002); Glyoxylate And Dicarboxylate Metabolism Pathway (383); Glyoxylate Metabolism Pathway (160990); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169)

ncbi summary :

This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I primary hyperoxaluria. [provided by RefSeq, Jul 2008]

uniprot summary :

AGXT: Defects in AGXT are the cause of hyperoxaluria primary type 1 (HP1); also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and the progressive accumulation of insoluble calcium oxalate in the kidney and urinary tract. Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Protein type: Motility/polarity/chemotaxis; Amino Acid Metabolism - alanine, aspartate and glutamate; Transferase; Mitochondrial; Amino Acid Metabolism - glycine, serine and threonine; EC 2.6.1.51; EC 2.6.1.44. Chromosomal Location of Human Ortholog: 2q37.3. Cellular Component: peroxisomal matrix; mitochondrial matrix; peroxisome. Molecular Function: amino acid binding; protein binding; protein homodimerization activity; alanine-glyoxylate transaminase activity; serine-pyruvate transaminase activity; transaminase activity; pyridoxal phosphate binding; receptor binding. Biological Process: pyruvate biosynthetic process; response to cAMP; glyoxylate catabolic process; glyoxylate metabolic process; glycine biosynthetic process, by transamination of glyoxylate; response to glucocorticoid stimulus; oxalic acid secretion; L-cysteine catabolic process; L-alanine catabolic process. Disease: Hyperoxaluria, Primary, Type I

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage