Human BAG family molecular chaperone regulator 4, BAG4 ELISA Kit | MyBioSource MBS9339475 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human BAG family molecular chaperone regulator 4, BAG4 ELISA Kit

catalog :

MBS9339475

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS9339475

products type :

ELISA Kit

products full name :

Human BAG family molecular chaperone regulator 4, BAG4 ELISA Kit

products short name :

BCL2-associated athanogene 4

products name syn :

Human BAG family molecular chaperone regulator 4 (BAG4) ELISA kit; BAG-4; SODD; BAG-family molecular chaperone regulator-4; silencer of death domains; BCL2-associated athanogene 4

other names :

BAG family molecular chaperone regulator 4 isoform 2; BAG family molecular chaperone regulator 4; BAG family molecular chaperone regulator 4; silencer of death domains; bcl-2-associated athanogene 4; BAG-family molecular chaperone regulator-4; BCL2-associated athanogene 4; Bcl-2-associated athanogene 4; Silencer of death domains

products gene name :

BAG4

other gene names :

BAG4; BAG4; SODD; BAG-4; SODD; BAG-4

uniprot entry name :

BAG4_HUMAN

reactivity :

Human

specificity :

No significant cross-reactivity or interference between Human BAG4 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 31.2 ng/ml - 1000 ng/ml. Sensitivity: 5.0 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated BAG4 concentrations in Human serum, plasma and other body fluids. Using Purified Human BAG4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add BAG4 and BAG4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of BAG4 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated BAG4 concentrations in Human serum, plasma and other body fluids. Using Purified Human BAG4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add BAG4 and BAG4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of BAG4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

325910869

ncbi acc num :

NP_001191807.1

ncbi gb acc num :

NM_001204878.1

uniprot acc num :

O95429

ncbi mol weight :

49,594 Da

ncbi pathways :

Ceramide Signaling Pathway (138023); HIV-1 Nef: Negative Effector Of Fas And TNF-alpha Pathway (137995); TNF Receptor Signaling Pathway (138053); TNF-alpha/NF-kB Signaling Pathway (198884)

ncbi summary :

The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. This protein was found to be associated with the death domain of tumor necrosis factor receptor type 1 (TNF-R1) and death receptor-3 (DR3), and thereby negatively regulates downstream cell death signaling. The regulatory role of this protein in cell death was demonstrated in epithelial cells which undergo apoptosis while integrin mediated matrix contacts are lost. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011]

uniprot summary :

BAG4: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Prevents constitutive TNFRSF1A signaling. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Apoptosis. Chromosomal Location of Human Ortholog: 8p11.23. Cellular Component: plasma membrane; nucleus; cytosol. Molecular Function: protein binding; chaperone binding; receptor signaling protein activity. Biological Process: positive regulation of protein kinase B signaling cascade; positive regulation of cell adhesion; positive regulation of actin filament polymerization; protein folding; protein heterooligomerization; positive regulation of stress fiber formation; positive regulation of peptidyl-serine phosphorylation; negative regulation of apoptosis

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage