catalog number :
MBS933945
products type :
ELISA Kit
products full name :
Rat Epoxide hydrolase 1, EPHX1 ELISA Kit
products short name :
epoxide hydrolase 1, microsomal (xenobiotic)
products name syn :
Rat Epoxide hydrolase 1 (EPHX1) ELISA kit; EPHX; EPOX; MEH; epoxide hydratase; epoxide hydrolase 1; epoxide hydrolase 1; microsomal (xenobiotic)
other names :
epoxide hydrolase 1; Epoxide hydrolase 1; epoxide hydrolase 1; epoxide hydratase; microsomal epoxide hydrolase; Epoxide hydratase; Microsomal epoxide hydrolase
products gene name :
EPHX1
other gene names :
EPHX1; EPHX1
uniprot entry name :
HYEP_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat EPHX1. No significant cross-reactivity or interference between Rat EPHX1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 3: Aromatic hydrocarbons catabolism
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EPHX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EPHX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EPHX1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EPHX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001075744.1
ncbi gb acc num :
NM_001082275.1
ncbi mol weight :
52,512 Da
uniprot summary :
Function: Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. Catalytic activity: Cis-stilbene oxide + H2O = (+)-(1R,2R)-1,2-diphenylethane-1,2-diol.
size4 :
10x96-Strip-Wells