catalog number :
MBS933881
products type :
ELISA Kit
products full name :
Mouse Bone marrow proteoglycan, PRG2 ELISA Kit
products short name :
proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein)
products name syn :
Mouse Bone marrow proteoglycan (PRG2) ELISA kit; BMPG; MBP; MBP1; MGC14537; bone-marrow proteoglycan; eosinophil granule major basic protein; eosinophil major basic protein; natural killer cell activator; proteoglycan 2; proteoglycan 2 preproprotein; proteoglycan 2; bone marrow (natural killer cell activator; eosinophil granule major basic protein)
other names :
bone marrow proteoglycan; Bone marrow proteoglycan; bone marrow proteoglycan; BMPG; major basic protein 1; proteoglycan 2, bone marrow; Proteoglycan 2Cleaved into the following chain:Eosinophil granule major basic protein; EMBP; MBP
products gene name :
PRG2
other gene names :
Prg2; Prg2; MBP; mMBP; mMBP-1; Mbp-1; BMPG; EMBP; MBP
uniprot entry name :
PRG2_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse PRG2. No significant cross-reactivity or interference between Mouse PRG2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.9 ng/ml-250 ng/ml. Sensitivity: 0.98 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Immunity
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRG2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_032946.1
ncbi gb acc num :
NM_008920.4
ncbi mol weight :
24,255 Da
ncbi pathways :
Asthma Pathway (83313); Asthma Pathway (532)
uniprot summary :
PRG2: Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA. Protein type: Secreted, signal peptide; Secreted; Vesicle. Molecular Function: carbohydrate binding. Biological Process: positive regulation of interleukin-4 production; negative regulation of interleukin-10 production; immune system process; defense response to bacterium; immune response; defense response to nematode
size4 :
10x96-Strip-Wells